US 11,053,184 B2
Downstream processing of fatty alcohol compositions produced by recombinant host cells
Myong K. Ko, South San Francisco, CA (US); Haibo Wang, South San Francisco, CA (US); Patricia J. Cole, South San Francisco, CA (US); Perry Y. Liao, South San Francisco, CA (US); and Simon Li, South San Francisco, CA (US)
Assigned to GENOMATICA, INC., San Diego, CA (US)
Appl. No. 14/773,149
Filed by GENOMATICA, INC., San Diego, CA (US)
PCT Filed Mar. 7, 2014, PCT No. PCT/US2014/021776
§ 371(c)(1), (2) Date Sep. 4, 2015,
PCT Pub. No. WO2014/138590, PCT Pub. Date Sep. 12, 2014.
Claims priority of provisional application 61/774,375, filed on Mar. 7, 2013.
Prior Publication US 2016/0009617 A1, Jan. 14, 2016
Int. Cl. C07C 29/88 (2006.01); C07C 29/17 (2006.01)
CPC C07C 29/88 (2013.01) [C07C 29/172 (2013.01)] 16 Claims
1. A method of purifying a fatty alcohol (FALC), comprising:
(a) providing a starting material comprising FALC and saponifiable impurities,
wherein said saponifiable impurities comprise free fatty acid (FFA), fatty-fatty ester (FFE), and carbonyl-containing compounds;
wherein the starting material comprises (a) at least 0.1 to 0.5 weight percent FFA or (b) at least 0.1 to 0.5 weight percent FFE; and
wherein the starting material is a fatty alcohol composition produced by a recombinant E. coli host cell grown in a fermentation broth;
(b) adding a strong base comprising 0.3 to 0.6 weight percent sodium hydroxide (NaOH) to said starting material to generate a first mixture; and
(c) evaporating FALC from said first mixture to generate a second mixture enriched for FALC, wherein the FALC in the second mixture is about 98% pure.