US 11,052,395 B2
Lateral cavity acoustic transducer (LCAT) for shear-induced cell transfection
Abraham P. Lee, Irvine, CA (US); Mohammad Aghaamoo, Irvine, CA (US); Xuan Li, Irvine, CA (US); Neha Garg, Irvine, CA (US); and Yu-Hsi Chen, Irvine, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Aug. 21, 2019, as Appl. No. 16/547,152.
Claims priority of provisional application 62/720,829, filed on Aug. 21, 2018.
Prior Publication US 2020/0061618 A1, Feb. 27, 2020
Int. Cl. B01L 3/00 (2006.01); C12N 15/87 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502769 (2013.01); C12N 15/87 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/18 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0809 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0436 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A system (100) for intracellular delivery of an exogenous material, the system comprising:
a. a microfluidic platform (110) comprising a main microfluidic channel (120), and one or more lateral cavity acoustic transducers (LCATs) (130), wherein the one or more LCATs (130) are dead-end side channels coupled to the main microfluidic channel (120), wherein the microfluidic platform (110) is coupled to an external acoustic source (140); and
b. a fluid (150) disposed through the main microfluidic channel (120), said fluid (150) comprising a cell (160) and an exogenous material (170), wherein the fluid (150) intersects the LCATs (130) to form one or more gas-liquid interfaces (180);
wherein the LCATs (130) are configured to oscillate the gas-liquid interfaces (160) to produce a plurality of microstreaming vortices (190), and wherein the vortices (190) trap cells (160) and exogenous material (170) therein, thereby shear-inducing mechanical deformation of the cells (160), and allowing for delivery of the exogenous material (170) into the cell (160); and
wherein the shear-induced mechanical deformation is configured to deform the cell membrane and cause it to be permeable to the exogenous material.