US 11,052,330 B2
Simple LAMP PCR design for low resource setting and minimal environmental footprint
Robert Beitle, Fayetteville, AR (US); Christa N. Hestekin, Fayetteville, AR (US); Ahmed Elmasheiti, Fayetteville, AR (US); Kimberly Cribbs, Pea Ridge, AR (US); Michael Rienisch, Fayetteville, AR (US); Bryce Cameron Jones, Flower Mound, TX (US); Allysa Swearingen, Farmington, AR (US); Brandon Hart, Fort Smith, AR (US); Kevin Clark, Springdale, AR (US); and Vicki Thompson, Springdale, AR (US)
Assigned to Board Of Trustees Of The University Of Arkansas, Little Rock, AR (US); and NowDiagnostics, Inc., Springdale, AR (US)
Filed by BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS, Little Rock, AR (US); and NowDiagnostics, Springdale, AR (US)
Filed on Mar. 31, 2017, as Appl. No. 15/476,753.
Claims priority of provisional application 62/316,345, filed on Mar. 31, 2016.
Prior Publication US 2017/0283858 A1, Oct. 5, 2017
Int. Cl. C12Q 1/68 (2018.01); B01D 21/26 (2006.01); C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); G01N 1/30 (2006.01); B01L 7/00 (2006.01); B01L 9/06 (2006.01); C12Q 1/6844 (2018.01); G01N 31/22 (2006.01)
CPC B01D 21/262 (2013.01) [B01D 21/26 (2013.01); B01L 7/00 (2013.01); B01L 9/06 (2013.01); C12N 15/1006 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6844 (2013.01); G01N 1/30 (2013.01); B01L 2200/10 (2013.01); B01L 2200/147 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/18 (2013.01); B01L 2300/1877 (2013.01); G01N 31/22 (2013.01); G01N 2001/302 (2013.01)] 20 Claims
1. A method for testing a material comprising the steps of:
obtaining a sample of solution of cells forming a first analyte and placing said sample in an enclosure;
concentrating said cells in said first analyte;
lysing said cells;
extracting DNA from said cells to form a second analyte;
applying heat at predetermined temperature to said enclosure to amplify said DNA in said second analyte;
using a phase change material that melts at a predetermined temperature to maintain said predetermined temperature during said heating; and
detecting said DNA.