US 11,052,165 B2
Method for virus clearance
Andreas Castan, Uppsala (SE); and Colin R. Tuohey, Marlborough, MA (US)
Assigned to Global Life Sciences Solutions USA LLC, Marlborough, MA (US)
Filed by Global Life Sciences Solutions USA LLC, Marlborough, MA (US)
Filed on Sep. 28, 2017, as Appl. No. 15/718,831.
Application 15/718,831 is a continuation in part of application No. 15/561,826, previously published as PCT/EP2016/057971, filed on Apr. 12, 2016.
Claims priority of provisional application 62/149,813, filed on Apr. 20, 2015.
Prior Publication US 2018/0015189 A1, Jan. 18, 2018
Int. Cl. A61L 2/00 (2006.01); C12N 5/00 (2006.01); C12N 13/00 (2006.01)
CPC A61L 2/0017 (2013.01) [A61L 2/007 (2013.01); A61L 2/0023 (2013.01); A61L 2/0035 (2013.01); C12N 5/0018 (2013.01); C12N 13/00 (2013.01); C12N 2500/32 (2013.01); C12N 2500/34 (2013.01); C12N 2500/38 (2013.01); C12N 2523/00 (2013.01)] 20 Claims
1. A method for virus clearance of a cell culture medium, comprising the steps of:
i) providing a bulk medium portion, comprising amino acids and glucose;
ii) providing a first additive portion, comprising vitamins in aqueous solution;
iii) subjecting the bulk medium portion to a high temperature short time treatment (HTST);
iv) passing the first additive portion through a virus retentive ultrafilter, wherein or the virus retentive ultrafilter has a molecular weight (Mw) cut-off value below 100 kDa; and
v) after steps iii) and iv), mixing the bulk medium portion with the first additive portion to obtain a cell culture medium cleared from viruses.