US 11,052,044 B2
In situ lipid synthesis for protein reconstitution
Roberto Javier Brea Fernandez, San Diego, CA (US); Christian Cole, Del Mar, CA (US); Neal K. Devaraj, La Jolla, CA (US); Brent Lyda, San Diego, CA (US); and Roger Sunahara, San Diego, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 16/489,783
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Mar. 1, 2018, PCT No. PCT/US2018/020512
§ 371(c)(1), (2) Date Aug. 29, 2019,
PCT Pub. No. WO2018/160876, PCT Pub. Date Sep. 7, 2018.
Claims priority of provisional application 62/466,236, filed on Mar. 2, 2017.
Prior Publication US 2020/0022915 A1, Jan. 23, 2020
Int. Cl. A61K 9/127 (2006.01); C07F 9/10 (2006.01); C07H 15/14 (2006.01); C07K 14/705 (2006.01)
CPC A61K 9/1277 (2013.01) [A61K 9/127 (2013.01); C07F 9/106 (2013.01); C07H 15/14 (2013.01); C07K 14/705 (2013.01)] 15 Claims
OG exemplary drawing
1. A method for forming a lipid membrane comprising a membrane protein, the method comprising:
adding a reactive maltoside surfactant comprising a reactive thioester moiety to a first solution which comprises the membrane protein; thereby forming a second solution which comprises the reactive maltoside surfactant and the membrane protein; and
(ii) adding a cysteine-functionalized phosphatidylcholine compound to the second solution, thereby forming a synthetic phospholipid; wherein the synthetic phospholipid forms a lipid membrane comprising the membrane protein.