US 11,051,469 B2
Polyploid rice photo-thermo-sensitive genetic male sterile line and breeding method thereof
Detian Cai, Hubei (CN); Xianhua Zhang, Hubei (CN); Zhaojian Song, Hubei (CN); Wei Wang, Hubei (CN); Yuhua Liu, Hubei (CN); and Yuchi He, Hubei (CN)
Assigned to WUHAN POLYPLOID BIOTECHNOLOGY CO., LTD, Hubei (CN); and HUBEI UNIVERSITY, Hubei (CN)
Filed by WUHAN POLYPLOID BIOTECHNOLOGY CO., LTD, Hubei (CN); and HUBEI UNIVERSITY, Hubei (CN)
Filed on Mar. 20, 2018, as Appl. No. 15/926,156.
Application 15/926,156 is a continuation of application No. PCT/CN2017/095930, filed on Aug. 4, 2017.
Claims priority of application No. 201610864024.0 (CN), filed on Sep. 29, 2016.
Prior Publication US 2018/0206425 A1, Jul. 26, 2018
Int. Cl. A01H 1/04 (2006.01); A01H 1/02 (2006.01); A01H 4/00 (2006.01); A01H 1/06 (2006.01); A01H 6/46 (2018.01); A01H 5/10 (2018.01); A01H 1/08 (2006.01)
CPC A01H 1/04 (2013.01) [A01H 1/02 (2013.01); A01H 1/06 (2013.01); A01H 1/08 (2013.01); A01H 4/008 (2013.01); A01H 5/10 (2013.01); A01H 6/4636 (2018.05)] 4 Claims
 
1. A breeding method of a polyploid rice photo-thermo-sensitive genetic male sterile line, characterized in that the breeding process comprises the steps:
(a) determining a diploid rice line with photo-thermo-sensitive genetic male sterility or polyploid meiosis stability (PMeS) characteristic as a parent;
(b) carrying out hybridization on a diploid photo-thermo-sensitive genetic male sterile line as the female parent and a diploid rice with the PMeS characteristic as the male parent to prepare a diploid hybrid rice, wherein the diploid photo-thermo-sensitive genetic male sterile line is HD9802S, and sample of seed of said HD9802S is deposited under CCTCC No: P202106; and the diploid rice with the PMeS characteristic is HN2026-2X, and sample of seed of said HN2026-2X is deposited under CCTCC No: P202105;
(c) carrying out doubling culture on a young ear of the diploid hybrid rice in step (b) into a tetraploid hybrid rice;
(d) back-crossing the tetraploid hybrid rice in step (c) with a tetraploid photo-thermo-sensitive genetic male sterile line, and selecting a tetraploid photo-thermo-sensitive genetic male sterile rice from the back-crossed progeny;
(e) self-crossing the tetraploid photo-thermo-sensitive genetic male sterile rice selected in step (d) during a low-temperature short-day fertile period, then carrying out composite hybridization on the self-crossed progeny with another tetraploid rice line having PMeS gene, self-crossing the composite hybridization progeny, and selecting a tetraploid photo-thermo-sensitive genetic male sterile rice, wherein said another tetraploid rice for the composite hybridization is a tetraploid rice line with the PMeS gene of HN164-4X, and sample of seed of said HN164-4X is deposited under CCTCC No: P202101;
(f) continuously self-crossing the tetraploid photo-thermo-sensitive genetic male sterile rice selected in step (e) by multiple generations during the low-temperature and short-day fertile period;
(g) detecting the stability of tetraploid photo-thermo-sensitive genetic male sterile rice in step (f) after multiple generations of continuous self-crossing, including the number of chromosomes 4X=48, morphological characteristics of anther sterility, pollen fertility, stigma traits, fertility conversion under a light-temperature condition, and heterogamety and heterosis after hybridization with tetraploid restorer lines; and
(h) determining stable and consistent tetraploid photo-thermo-sensitive genetic male sterile rice as the polyploid rice photo-thermo-sensitive genetic male sterile line, wherein the polyploid rice photo-thermo-sensitive genetic male sterile line is named as PS006, and a representative sample of seed of said PS006 is deposited under CCTCC No: P202107.