US 12,347,665 B2
Rapid identification and sequence analysis of intact proteins in complex mixtures
Donald F. Hunt, Charlottesville, VA (US); Ann M. English, Andover, MA (US); Scott A. Ugrin, Charlottesville, VA (US); Dina L. Bai, Charlottesville, VA (US); Jeffrey Shabanowitz, Charlottesville, VA (US); and John Edward Philip Syka, Charlottesville, VA (US)
Assigned to UNIVERSITY OF VIRGINIA PATENT FOUNDATION, Charlottesville, VA (US); and THERMO FINNIGAN LLC, San Jose, CA (US)
Filed by UNIVERSITY OF VIRGINIA PATENT FOUNDATION, Charlottesville, VA (US); and THERMO FINNIGAN LLC, San Jose, CA (US)
Filed on Dec. 9, 2022, as Appl. No. 18/063,975.
Application 18/063,975 is a continuation of application No. 16/307,615, granted, now 11,538,675, previously published as PCT/US2017/035953, filed on Jun. 5, 2017.
Claims priority of provisional application 62/346,268, filed on Jun. 6, 2016.
Prior Publication US 2023/0178350 A1, Jun. 8, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. H01J 49/00 (2006.01); G01N 33/68 (2006.01); H01J 49/04 (2006.01); H01J 49/42 (2006.01)
CPC H01J 49/0072 (2013.01) [G01N 33/6848 (2013.01); H01J 49/0031 (2013.01); H01J 49/04 (2013.01); H01J 49/4295 (2013.01); G01N 2560/00 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for mass spectrometry analysis, comprising:
introducing ions into a reaction cell, the ions including analyte precursor ions and reagent ions;
applying radio-frequency (RF) voltages to electrodes of the reaction cell to generate an RF field, such that ions present within the reaction cell have oscillatory motion frequencies that are primarily determined by their mass-to-charge ratios; and
while applying the RF voltages:
generating a first auxiliary AC dipole field, oriented in a first dimension, having at least one component frequency selected to kinetically excite analyte product ions formed by reaction of the analyte precursor ions with the reagent ions;
generating a second auxiliary AC dipole field oriented in a second dimension orthogonal to the first dimension, the second auxiliary dipole field having at least one component frequency selected to kinetically excite reagent ions, and wherein the component frequencies of the first and second auxiliary AC dipole fields differ from one another.