US 12,345,722 B1
Liquid handling calibration device and associated method of calibration
Philippe Renaud, Préverenges (CH); Daniel Bertrand, Anières (CH); Andreas Möller, Tübingen (DE); and Karl-Heinz Boven, Wannweil (DE)
Assigned to UNIPIX SÀRL, Preverenges (CH)
Filed by UniPix Sàrl, Préverenges (CH)
Filed on Dec. 31, 2024, as Appl. No. 19/006,930.
Claims priority of application No. 24172380 (EP), filed on Apr. 25, 2024.
Int. Cl. G06F 11/30 (2006.01); B01L 3/02 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01)
CPC G01N 35/00693 (2013.01) [B01L 3/021 (2013.01); G01N 35/1016 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A volumetric displacement calibration device comprising:
at least one opening configured to adapt at least one suction system, comprising a tip, having a volume, connected to a shaft comprising a volume change mechanism configured to be movable between a distal position and a proximal position closer to said opening than said distal position;
at least one main chamber and one reference chamber, connected, by a conduit, to said at least one main chamber;
a valve disposed on said conduit and configured to be switchable between an open position, in which said main chamber is in fluid communication with said reference chamber, and a closed position in which said main chamber and said reference chamber are not in fluid communication, the reference chamber having a defined volume Vref when said valve is closed;
at least one first pressure sensor connected to said main chamber, the at least one first pressure sensor being configured to measure:
at least one pressure P00 measured in said main chamber when the volume change mechanism is in said distal position and the valve is opened,
at least one pressure P0 measured in said main chamber, when the volume change mechanism is in the distal position and the valve is closed,
at least one pressure P1 measured in said main chamber when the volume change mechanism is in said proximal position and the valve is closed, and
at least one pressure P2 measured in said main chamber, when the volume change mechanism is in the proximal position and the valve is opened;
one second pressure sensor connected to said reference chamber and configured to measure:
at least one pressure P00y measured in said reference chamber when the volume change mechanism is in the distal position and the valve is opened, and
at least one pressure P0y measured in said reference chamber when the volume change mechanism is in the distal position, after closure of the valve; and
a first calculator configured to calculate an internal volume which is the sum of the volume of said tip, a residual volume of the shaft when the volume change mechanism is in the proximal position, and a volume of said main chamber, said internal volume being determined by:
Vo=Vref*(P2−Pc)/(P1−P2),
wherein Pc=P00+(P0y−P00y),
Vo is the internal volume, and
Vref is the defined volume of the reference chamber when the valve is closed.