US 12,345,710 B2
Marker for diagnosing colorectal cancer and method for providing information required for diagnosis of colorectal cancer
Eun Suk Kang, Seoul (KR); Hee Cheol Kim, Seoul (KR); and Jae Won Yun, Seoul (KR)
Assigned to SAMSUNG LIFE PUBLIC WELFARE FOUNDATION, Seoul (KR)
Appl. No. 17/594,925
Filed by SAMSUNG LIFE PUBLIC WELFARE FOUNDATION, Seoul (KR)
PCT Filed Apr. 29, 2020, PCT No. PCT/KR2020/005778
§ 371(c)(1), (2) Date Nov. 3, 2021,
PCT Pub. No. WO2020/226366, PCT Pub. Date Nov. 12, 2020.
Claims priority of application No. 10-2019-0052556 (KR), filed on May 3, 2019.
Prior Publication US 2022/0221462 A1, Jul. 14, 2022
Int. Cl. G01N 33/574 (2006.01); G01N 1/30 (2006.01)
CPC G01N 33/57446 (2013.01) [G01N 1/30 (2013.01); G01N 2440/14 (2013.01)] 11 Claims
 
1. A method for providing information required for the diagnosis of colorectal cancer, the method comprising:
measuring the level of signal transducer and activator of transcription 3 (STAT3) phosphorylation in helper T cells (Th cells) contained in a first sample; and
measuring the level of STAT3 phosphorylation in cytotoxic T cells (Tc cells) contained in a second sample,
wherein the first sample is prepared by:
a first mononuclear cell isolation step of isolating mononuclear cells contained in blood;
a first cytokine treatment step of treating the isolated mononuclear cells with IL-10; and
a first phosphorylation staining step of performing phosphorylation staining,
wherein the second sample is prepared by:
a second mononuclear cell isolation step of isolating mononuclear cells contained in blood;
a second cytokine treatment step of treating the isolated mononuclear cells with IL-6; and
a second phosphorylation staining step of performing phosphorylation staining.