US 12,345,707 B2
Fluorescence imaging-based device for detecting microorganisms and method for manufacturing the same
Nae Eung Lee, Suwon-si (KR); and Won Il Lee, Suwon-si (KR)
Assigned to Research & Business Foundation Sungkyunkwan University, Suwon-si (KR)
Filed by RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIVERSITY, Suwon-si (KR)
Filed on Dec. 14, 2020, as Appl. No. 17/120,396.
Claims priority of application No. 10-2019-0166599 (KR), filed on Dec. 13, 2019.
Prior Publication US 2021/0181197 A1, Jun. 17, 2021
Int. Cl. G01N 33/569 (2006.01); B01L 3/00 (2006.01); C12Q 1/06 (2006.01); G01N 21/64 (2006.01); G01N 33/533 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/569 (2013.01) [B01L 3/5023 (2013.01); C12Q 1/06 (2013.01); G01N 21/6428 (2013.01); G01N 33/533 (2013.01); G01N 33/54326 (2013.01); B01L 2300/0809 (2013.01); G01N 2021/6439 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A fluorescence imaging-based device for detecting microorganisms, comprising:
a magnet;
a substrate on which the magnet is disposed;
a microfluidic channel layer, which is disposed in an annular configuration above the magnet, having a microfluidic channel formed on a surface thereof and having a separation reaction space configured to hold a detection sample and a high-viscosity liquid at a radial center of the annular configuration; and
an absorption layer disposed on the microfluidic channel layer and having an empty space penetrating the absorption layer,
wherein, in a plan view, an area of the empty space penetrating the absorption layer is greater than an area of the separation reaction space and the same or smaller than an area of the microfluidic channel layer, and the absorption layer and the microfluidic channel layer are in direct contact with each other,
when the detection sample is injected into the separation reaction space in the microfluidic channel layer, and the detection sample comprises free magnetic particles and magnetic particle-conjugated fluorescence-labeled microorganisms,
the microfluidic channel layer is configured so that the free magnetic particles in the detection sample reach the absorption layer through the microfluidic channel formed in the microfluidic channel layer along with the high-viscosity liquid, and then are absorbed by the absorption layer to be removed from the separation reaction space, and
the magnetic particle-conjugated fluorescence-labeled microorganisms in the detection sample are captured by the magnet in the separation reaction space.