US 12,345,706 B2
Detection kit and preparation method thereof and a detection method for novel coronavirus
Li Wang, Beijing (CN); and Lang Chen, Beijing (CN)
Assigned to BEIJING JINWOFU BIOENGINEERING TECHNOLOGY CO., LTD, Beijing (CN)
Appl. No. 17/759,279
Filed by BEIJING JINWOFU BIOENGINEERING TECHNOLOGY CO., LTD, Beijing (CN)
PCT Filed Sep. 22, 2021, PCT No. PCT/CN2021/119553
§ 371(c)(1), (2) Date Jul. 21, 2022,
PCT Pub. No. WO2022/068641, PCT Pub. Date Apr. 7, 2022.
Claims priority of application No. 202011069524.8 (CN), filed on Sep. 30, 2020.
Prior Publication US 2023/0077887 A1, Mar. 16, 2023
Int. Cl. G01N 33/543 (2006.01); G01N 33/569 (2006.01)
CPC G01N 33/54388 (2021.08) [G01N 33/56983 (2013.01); G01N 2333/165 (2013.01)] 10 Claims
 
1. A method of making a single-person antigen test strip card, comprising:
a plastic substrate;
a bibulous paper;
an immune nitrocellulose membrane; and
an immune microsphere pad; wherein the immune microsphere pad, the immune nitrocellulose membrane and the bibulous paper are disposed on the plastic substrate; the immune microsphere pad comprises severe respiratory syndrome coronavirus 2 (SARS-CoV-2) monoclonal antibody 1 including latex microspheres; the immune nitrocellulose membrane includes a test line and a quality control line, the test line comprising severe respiratory syndrome coronavirus (SARS-CoV-2) monoclonal antibody 2 and the quality control line comprising goat anti-mouse immunoglobulin G polyclonal antibody wherein the method comprises:
A) preparing a first solution comprising immune microspheres by labeling an amount of severe respiratory syndrome coronavirus (SARS-CoV-2) monoclonal antibody 1 using an amount of latex microspheres;
B) preparing the immune microsphere pad by diluting the first solution and coating a release pad using the diluted first solution;
C) preparing a second solution comprising severe respiratory syndrome coronavirus (SARS-CoV-2) monoclonal antibody 2, and preparing a third solution comprising goat anti-mouse immunoglobulin G;
D) preparing the immune nitrocellulose membrane by spraying the third solution onto the quality control line and by spraying the second solution onto the test line;
E) drying the immune microsphere pad and drying the immune nitrocellulose membrane;
F) forming a plate by pasting the immune microsphere pad, the immune nitrocellulose membrane and the bibulous paper onto the plastic substrate; and
G obtaining the single-person antigen test trip card by pasting a piece of handle paper and polyurethane glue on the plate, cutting the plate into two or more reagent strips, assembling the two or more reagent strips into a card, putting the assembled card and a desiccant into an aluminum foil bag, and sealing the aluminum foil bag.