US 12,345,704 B2
Systems for improving the dynamic range of biological assays
Yong Song, Pomona, CA (US)
Assigned to AimPlex Biosciences, Inc., Pomona, CA (US)
Filed by AimPlex Biosciences, Inc., Pomona, CA (US)
Filed on Jun. 13, 2022, as Appl. No. 17/839,210.
Application 17/839,210 is a continuation of application No. 16/115,703, filed on Aug. 29, 2018, granted, now 11,391,735.
Claims priority of provisional application 62/554,889, filed on Sep. 6, 2017.
Prior Publication US 2022/0308050 A1, Sep. 29, 2022
Int. Cl. G01N 33/543 (2006.01); C12M 1/12 (2006.01); C12M 3/06 (2006.01)
CPC G01N 33/54386 (2013.01) [C12M 23/16 (2013.01); C12M 25/01 (2013.01); B01L 2200/027 (2013.01); B01L 2300/0819 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A system for performing a biological assay comprising:
a plurality of first discrete test sites;
a sensor for obtaining at least a first signal from each of the plurality of discrete test sites; and
encoded instructions for performing a first biological assay directed to a first analyte on a sample using the plurality of first discrete test sites, wherein the first biological assay provides a change in the first signal indicative of the presence of the first analyte, comprising:
deriving a plurality of quantities for the first signal, wherein each of the plurality of quantities is associated with the first signal observed from an individual first discrete test sites of a first population of the first discrete test sites;
determining, using at least a portion of the plurality of quantities, a first proportion, wherein the first proportion comprises an enumeration of the first discrete test sites indicating the presence of the analyte;
calculating a statistical distribution of the plurality of quantities derived from the first discrete test sites to derive a statistical value for the first signal; and
quantifying the mass of the first analyte in the sample using the first proportion when the first proportion falls within a first cutoff range of values for the first proportion, and using the statistical value of the first signal when the first proportion falls outside of the first cutoff range of values for the first proportion.