US 12,345,677 B2
Integrated circuit with 2D FETs for direct and indirect target signal measurement
Kiana Aran, Pasadena, CA (US); Brett Goldsmith, San Diego, CA (US); Alexander Kane, Santa Cruz, CA (US); and Regis Peytavi, Costa Mesa, CA (US)
Assigned to Cardea Bio, Inc., San Diego, CA (US)
Filed by Cardea Bio, Inc., San Diego, CA (US)
Filed on May 20, 2022, as Appl. No. 17/749,993.
Application 17/749,993 is a continuation in part of application No. 17/342,284, filed on Jun. 8, 2021, abandoned.
Claims priority of provisional application 63/191,307, filed on May 20, 2021.
Claims priority of provisional application 63/314,270, filed on Feb. 25, 2022.
Claims priority of provisional application 63/343,019, filed on May 17, 2022.
Claims priority of provisional application 63/036,772, filed on Jun. 9, 2020.
Prior Publication US 2022/0365024 A1, Nov. 17, 2022
Int. Cl. G01N 27/414 (2006.01)
CPC G01N 27/4145 (2013.01) [G01N 27/4148 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A system for indirect target substance signal measurement comprising:
an integrated circuit comprising an array of 2D FETs, at least a portion of one or more selected instances of the 2D FETs in the array individually comprising:
a 2D transistor channel disposed on a substrate, the 2D transistor channel formed in a layer of 2D nanomaterial,
a gate area for receiving a volume of liquid comprising one or more chemical or biological target substances,
a conductive source electrically coupled to a first end of the 2D transistor channel,
a conductive drain electrically coupled to a second end of the 2D transistor channel, and
one or more layers of synthetic biopolymer specific binding agents adsorbed directly or indirectly to a top surface of the 2D transistor channel of the one or more selected instances of the 2D FETs in the array, wherein at least a portion of the one or more layers of synthetic biopolymer specific binding agents is selected to bind to a target substance in a liquid applied to the array of 2D FETs in contact with the top surface and in response to binding with the target substance to desorb from the 2D transistor channel of the one or more selected instances of the 2D FETs;
one or more environmentally non-reactive electrodes for biasing and/or measuring electrical characteristics of the liquid, the one or more environmentally non-reactive electrodes disposed on the substrate;
a measurement controller operable to apply one or more excitation conditions to the array of 2D FETs such that one or more output signals from the one or more selected instances of the 2D FETS of the array are affected by binding of the synthetic biopolymer specific binding agents to the target substance in the liquid causing the one or more layers of synthetic biopolymer specific binding agents to desorb from the 2D transistor channel of one or more selected instances of the 2D FETS to which they were adsorbed, and to perform one or more measurements of the one or more output signals affected by binding of the synthetic biopolymer specific binding agents; and
an analysis module operable to determine measurement vectors based on the one or more measurements, wherein at least a portion of the analysis module and the measurement controller individually comprises one or more of a set of hardware circuits, a set of programmable logic, and executable code stored on non-transitory computer-readable storage media.