US 12,345,671 B2
Analyte sensors for detecting asparagine and aspartate and methods of use thereof
Zenghe Liu, Alameda, CA (US); Barry S. Kreutz, Chicago, IL (US); Yagya Raj Ojha, Alameda, CA (US); Benjamin J. Feldman, Berkeley, CA (US); and Thomas P. Leary, Peoria, AZ (US)
Assigned to Abbott Diabetes Care Inc., Alameda, CA (US)
Filed by Abbott Diabetes Care Inc., Alameda, CA (US)
Filed on Dec. 23, 2021, as Appl. No. 17/561,505.
Claims priority of provisional application 63/132,200, filed on Dec. 30, 2020.
Prior Publication US 2022/0205944 A1, Jun. 30, 2022
Int. Cl. G01N 27/327 (2006.01); A61B 5/1486 (2006.01); C12N 9/06 (2006.01); C12N 9/82 (2006.01)
CPC G01N 27/3273 (2013.01) [A61B 5/1486 (2013.01); C12N 9/0022 (2013.01); C12N 9/82 (2013.01); G01N 27/3272 (2013.01); G01N 27/3274 (2013.01)] 13 Claims
OG exemplary drawing
 
1. An analyte sensor for detecting asparagine in vivo, the sensor comprising:
(i) at least a first working electrode;
(ii) at least one asparagine-responsive active area for detecting asparagine, the asparagine-responsive active area disposed upon a surface of the first working electrode, wherein the asparagine-responsive active area comprises aspartate oxidase and asparaginase; and
(iii) a mass transport limiting membrane permeable to asparagine that overcoats at least the asparagine-responsive active area,
wherein the sensor is configured to be partially inserted into a user's skin such that a distal portion of the sensor is in contact with interstitial fluid to detect asparagine in vivo.