US 12,345,645 B2
Sensor plant and method for identifying stressors in crops based on characteristics of sensor plants
Shely Aronov, San Francisco, CA (US); Roderick Kumimoto, San Francisco, CA (US); Nicholas Koshnick, San Francisco, CA (US); and Ari Kornfeld, San Francisco, CA (US)
Assigned to InnerPlant, Inc., Davis, CA (US)
Filed by InnerPlant, Inc., San Francisco, CA (US)
Filed on Feb. 25, 2022, as Appl. No. 17/680,978.
Application 17/680,978 is a continuation of application No. 17/479,965, filed on Sep. 20, 2021, granted, now 11,366,063.
Application 17/479,965 is a continuation of application No. 16/721,830, filed on Dec. 19, 2019.
Claims priority of provisional application 62/894,676, filed on Aug. 30, 2019.
Claims priority of provisional application 62/864,401, filed on Jun. 20, 2019.
Claims priority of provisional application 62/782,130, filed on Dec. 19, 2018.
Prior Publication US 2022/0178829 A1, Jun. 9, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/64 (2006.01); A01G 7/00 (2006.01); A01G 25/16 (2006.01); B64G 1/10 (2006.01); B64U 101/30 (2023.01); B64U 101/40 (2023.01); G06T 7/00 (2017.01); G06V 10/22 (2022.01)
CPC G01N 21/6486 (2013.01) [A01G 7/00 (2013.01); A01G 25/16 (2013.01); G01N 21/6456 (2013.01); G06T 7/0012 (2013.01); G06V 10/225 (2022.01); B64G 1/1021 (2013.01); B64G 1/1028 (2023.08); B64U 2101/30 (2023.01); B64U 2101/40 (2023.01)] 16 Claims
OG exemplary drawing
 
1. A method for identifying stressors in crops based on fluorescence of sensor plants comprising: accessing one or more images of at least one sensor plant in an agricultural environment, wherein the one or more images depict a set of downwelling light spectra and a set of upwelling light spectra corresponding to solar radiation, and the at least one sensor plant have been genetically modified to include a promoter-reporter pair-configured to express a fluorescent signal indicating presence of a stressor selected from a microbial, nematode, fungal, insect, or environmental stressor, the promoter-reporter pair comprising: a promoter gene configured to selectively express based on presence of the stressor; and a reporter gene-paired to the promoter gene and configured to express, to produce the fluorescent signal, responsive to expression of the promoter gene; accessing a reporter model linking solar-induced fluorescence measurements extracted from the one or more images of sensor plants to the presence of the stressor, based on fluorescent signals expressed by the at least one sensor plant;
and interpreting presence of the stressor at the at least one sensor plant based on the reporter model and the solar-induced fluorescence measurements extracted from the one or more images,
comprising extracting a solar-induced fluorescence measurement in a reporter fluorescent light spectrum associated with the fluorescent signal; and interpreting presence of the stressor at the at least one sensor plant based on the reporter model and the solar-induced fluorescence measurement, wherein the solar induced fluorescence measurements are associated with one or more wavelengths.