US 12,345,643 B2
Two-photon microscope
Hong-Seok Seo, Daejeon (KR); Dong Hoon Song, Daejeon (KR); and Chul Huh, Daejeon (KR)
Assigned to ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE, Daejeon (KR)
Filed by ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE, Daejeon (KR)
Filed on Dec. 27, 2022, as Appl. No. 18/088,958.
Claims priority of application No. 10-2022-0065261 (KR), filed on May 27, 2022.
Prior Publication US 2023/0384226 A1, Nov. 30, 2023
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6458 (2013.01) [G01N 2021/6463 (2013.01); G01N 2021/6484 (2013.01); G01N 2201/0612 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A two-photon microscope comprising:
a first laser light source configured to generate first laser light;
a second laser light source connected to the first laser light source and configured to generate second laser light having a longer wavelength than a wavelength of the first laser light;
an objective lens configured to provide the first laser light and the second laser light to a sample;
a sensor configured to detect first fluorescent light and second fluorescent light generated in the sample due to the first laser light and the second laser light; and
first and second code controllers provided between the first and second laser light sources and the objective lens and configured to switch the first laser light and the second laser light respectively using an orthogonal code method,
wherein the first laser light source comprises:
a first optical fiber;
a semiconductor saturable absorber mirror connected to one side of the first optical fiber;
a volume Bragg grating provided to another side of the first optical fiber; and
a first pump light source connected to the first optical fiber between the volume Bragg grating and the semiconductor saturable absorber mirror, the first pump light source configured to provide first pump light in the first optical fiber.