US 12,344,905 B2
Compositions and methods for detection of Candida auris
Ellen H. Fiss Hobart, Albany, CA (US); Jody Harris, Lafayette, CA (US); Andrew T. Hill, Livermore, CA (US); and Jingtao Sun, San Ramon, CA (US)
Assigned to Roche Molecular Systems, Inc., Pleasanton, CA (US)
Appl. No. 17/311,442
Filed by Roche Molecular Systems, Inc., Pleasanton, CA (US)
PCT Filed Dec. 3, 2019, PCT No. PCT/EP2019/083389
§ 371(c)(1), (2) Date Jun. 7, 2021,
PCT Pub. No. WO2020/114998, PCT Pub. Date Jun. 11, 2020.
Claims priority of provisional application 62/774,742, filed on Dec. 3, 2018.
Prior Publication US 2022/0017973 A1, Jan. 20, 2022
Int. Cl. C12Q 1/6895 (2018.01)
CPC C12Q 1/6895 (2013.01) 16 Claims
 
1. A method of detecting Candida auris (CA) in a sample that discriminates against Candida haemulonii, the method comprising:
performing an amplifying step comprising contacting the sample with a set of CA 5.8s/ITS2 rRNA gene primers to produce an amplification product if CA 5.8s/ITS2 rRNA nucleic acid is present in the sample;
performing a hybridizing step comprising contacting the amplification product with one or more detectable CA 5.8s/ITS2 rRNA gene probes; and
detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of CA in the sample and wherein the absence of the amplification product is indicative of the absence of CA in the sample;
wherein the set of CA 5.8s/ITS2 rRNA gene primers comprises a first oligonucleotide primer comprising a first nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, and a second oligonucleotide primer comprising a second nucleic acid sequence selected from the group consisting of SEQ ID NOs: 5-6, and 8; and
wherein the one or more detectable CA 5.8s/ITS2 rRNA gene oligonucleotide probe comprises a third nucleic acid sequence selected from the group consisting of SEQ ID NOs: 9-11, or the complement thereof, and
wherein the combination of the first oligonucleotide primer, the second oligonucleotide primer and the detectably labeled probe exhibit no detectable cross-reactivity with Candida haemulonii in a PCR amplification reaction.