US 12,344,903 B2
Method for predicting survival following Streptococcus iniae infection
Sergio Vela Avitúa, Vinterbro (NO); Carlos Lozano, Ås (NO); and Morten Rye, Grøa (NO)
Assigned to BENCHMARK GENETICS LIMITED, Sheffield (GB); and BENCHMARK GENETICS NORWAY AS, Bergen (NO)
Appl. No. 17/618,041
Filed by BENCHMARK GENETICS LIMITED, Sheffield (GB); and BENCHMARK GENETICS NORWAY AS, Bergen (NO)
PCT Filed Jun. 9, 2020, PCT No. PCT/GB2020/051394
§ 371(c)(1), (2) Date Dec. 10, 2021,
PCT Pub. No. WO2020/249939, PCT Pub. Date Dec. 17, 2020.
Claims priority of application No. 1908261 (GB), filed on Jun. 10, 2019.
Prior Publication US 2022/0307092 A1, Sep. 29, 2022
Int. Cl. C12Q 1/6883 (2018.01); C12Q 1/6888 (2018.01)
CPC C12Q 1/6888 (2013.01) [C12Q 2600/118 (2013.01); C12Q 2600/124 (2013.01); C12Q 2600/156 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method of producing a tilapia offspring resistant to Streptococcus iniae infection, the method comprising the steps of:
a) obtaining a tissue sample from a tilapia;
b) detecting in the sample from the tilapia the presence of alleles conferring resistance to infection by Streptococcus iniae at one or more DNA polymorphism selected from the group consisting of: NC_031973.1_7142946, NC_031973.1_9167743, NC_031973.1_6323968, NC_031973.1_7142916, NC_031973.1_7497722, NC_031973.1_7775443, NC_031973.1_7782524, NC_031973.1_9209387, NC_031973.1_9485417, and NC_031973.1_5545222;
c) selecting tilapia having alleles conferring resistance to infection by Streptococcus iniae from step b, wherein the alleles having resistance are selected from the group consisting of SEQ ID NOs: 1; 3; 5; 7; 9; 11; 13; 15; 17; and 19; and
d) subjecting the selected tilapia to aquaculture to produce tilapia offspring comprising resistance to infection by Streptococcus iniae.