	US 12,344,894 B2
	Methods for generating animals with desirable traits
Poothappillai K. Kasinathan, Brandon, SD (US); Hong Wei, Sioux Falls, SD (US); Mark F. Allan, Le Mars, IA (US); and David C. Faber, Sioux City, IA (US)
Assigned to TRANS OVA GENETICS, L.C., Sioux Center, IA (US)
Filed by TRANS OVA GENETICS, L.C., Sioux Center, IA (US)
Filed on Jan. 15, 2021, as Appl. No. 17/150,858.
Application 17/150,858 is a continuation of application No. 14/436,664, granted, now 10,927,411, previously published as PCT/US2013/065618, filed on Oct. 18, 2013.
Claims priority of provisional application 61/716,294, filed on Oct. 19, 2012.
Prior Publication US 2021/0189496 A1, Jun. 24, 2021
Int. Cl. C12Q 1/68 (2018.01); A01K 67/00 (2006.01); A01K 67/02 (2006.01); A01K 67/0273 (2024.01); C12N 5/07 (2010.01); C12Q 1/6883 (2018.01)

CPC C12Q 1/6883 (2013.01) [A01K 67/02 (2013.01); A01K 67/0273 (2013.01); A01K 2207/00 (2013.01); A01K 2227/101 (2013.01); A01K 2267/02 (2013.01); C12Q 2600/124 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01)]

3 Claims

1. A method for producing non-human mammalian progeny having a genetically-desirable trait, the method comprising:

(a) extracting a cell from each of a plurality of non-human embryos about 2 to 6 days after fertilization of the embryos;

(b) culturing the extracted cells from step (a) for about 2 to 6 days to prepare the cells for whole genome analysis;

(c) performing whole genome analysis on the cultured extracted cells to identify genetic markers associated with the genetically-desirable trait;

(d) selecting an embryo from the plurality of embryos when a cell extracted therefrom indicates the presence of one or more genetic markers associated with the genetically-desirable trait; and

(e) either (i) transferring the selected embryo to a recipient non-human mammal for the production of progeny, or (ii) using the selected embryo for cloning to produce progeny;

wherein, between steps (a) and (e), either (i) all embryos are cultured prior to the selection step (d), or (ii) only the selected embryo is cultured after the selection step (d).