US 12,344,892 B2
Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
Michael Schnall-Levin, San Francisco, CA (US); Michael Ybarra Lucero, South San Francisco, CA (US); Tarjei Sigurd Mikkelsen, Hayward, CA (US); Patrik Stahl, Stockholm (SE); Jonas Frisen, Stockholm (SE); Maja Marklund, Stockholm (SE); and Enric Llorens Bobadilla, Stockholm (SE)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Dec. 19, 2024, as Appl. No. 18/988,119.
Application 18/988,119 is a continuation of application No. 18/348,072, filed on Jul. 6, 2023, granted, now 12,270,077.
Application 18/348,072 is a continuation of application No. 17/933,347, filed on Sep. 19, 2022.
Application 17/933,347 is a continuation of application No. 17/688,241, filed on Mar. 7, 2022, granted, now 11,519,033.
Application 17/688,241 is a continuation of application No. 16/876,682, filed on May 18, 2020, abandoned.
Application 16/876,682 is a continuation of application No. PCT/US2019/048425, filed on Aug. 27, 2019.
Claims priority of provisional application 62/858,331, filed on Jun. 7, 2019.
Claims priority of provisional application 62/839,223, filed on Apr. 26, 2019.
Claims priority of provisional application 62/822,565, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,605, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,592, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,627, filed on Mar. 22, 2019.
Claims priority of provisional application 62/812,219, filed on Feb. 28, 2019.
Claims priority of provisional application 62/788,885, filed on Jan. 6, 2019.
Claims priority of provisional application 62/788,897, filed on Jan. 6, 2019.
Claims priority of provisional application 62/788,871, filed on Jan. 6, 2019.
Claims priority of provisional application 62/788,905, filed on Jan. 6, 2019.
Claims priority of provisional application 62/779,342, filed on Dec. 13, 2018.
Claims priority of provisional application 62/724,487, filed on Aug. 29, 2018.
Claims priority of provisional application 62/724,489, filed on Aug. 29, 2018.
Claims priority of provisional application 62/724,483, filed on Aug. 29, 2018.
Claims priority of provisional application 62/723,970, filed on Aug. 28, 2018.
Claims priority of provisional application 62/723,972, filed on Aug. 28, 2018.
Prior Publication US 2025/0129421 A1, Apr. 24, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6881 (2018.01); C12Q 1/48 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6841 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6881 (2013.01) [C12Q 1/48 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6841 (2013.01); C12Q 1/6874 (2013.01); C12Y 207/07 (2013.01)] 30 Claims
 
1. A method for spatially tagging a biological sample, the method comprising:
(a) providing:
(i) a first substrate comprising the biological sample, wherein the biological sample comprises a nucleic acid analyte; and
(ii) a second substrate comprising a hydrogel array, wherein the hydrogel array comprises a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises: (i) a spatial barcode and (ii) a capture domain;
(b) aligning the biological sample on the first substrate with the hydrogel array on the second substrate;
(c) releasing the capture probe from the hydrogel array such that the capture probe migrates to the biological sample and the capture domain of the capture probe hybridizes to the nucleic acid analyte of the biological sample; and
(d) extending the capture probe using the nucleic acid analyte as a template, thereby generating a spatially tagged nucleic acid product.