US 12,344,891 B2
Membrane bound nucleic acid nanopores
Stefan Howorka, London (GB); and Yongzheng Xing, London (GB)
Assigned to UCL Business Ltd, London (GB)
Appl. No. 17/265,244
Filed by UCL Business Ltd, London (GB)
PCT Filed Aug. 2, 2019, PCT No. PCT/GB2019/052181
§ 371(c)(1), (2) Date Feb. 2, 2021,
PCT Pub. No. WO2020/025974, PCT Pub. Date Feb. 6, 2020.
Claims priority of application No. 1812615 (GB), filed on Aug. 2, 2018.
Prior Publication US 2021/0301334 A1, Sep. 30, 2021
Int. Cl. C12Q 1/6869 (2018.01); C12Q 1/6837 (2018.01); B82Y 5/00 (2011.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6837 (2013.01); B82Y 5/00 (2013.01)] 25 Claims
 
1. A membrane-spanning nanopore, the nanopore comprising:
a) one or more polynucleotide strands that provide a scaffold component; and
b) a plurality of polynucleotide strands that provide a plurality of staple components;
wherein each of the plurality of staple components hybridise to the scaffold component;
wherein the orientation of a major portion of at least one polynucleotide strand comprised within the scaffold component is substantially parallel to a planar surface of a membrane as well as embedded within and substantially coplanar with the membrane;
wherein the nanopore defines a channel that is suitable for perforating the membrane;
the channel having a longitudinal axis extending along the centre thereof and a minimum internal dimension perpendicular to the longitudinal axis of at least about 3 nm.