US 12,344,881 B2
Genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid by taking glucose as substrate and applications thereof
Tianwei Tan, Beijing (CN); and Menglei Li, Beijing (CN)
Assigned to Beijing University of Chemical Technology, Beijing (CN)
Filed by Beijing University of Chemical Technology, Beijing (CN)
Filed on Sep. 5, 2024, as Appl. No. 18/825,195.
Application 18/825,195 is a continuation of application No. PCT/CN2024/095637, filed on May 28, 2024.
Claims priority of application No. 202311253745.4 (CN), filed on Sep. 27, 2023.
Prior Publication US 2024/0425889 A1, Dec. 26, 2024
Int. Cl. C12P 7/44 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12N 15/52 (2006.01); C12N 15/77 (2006.01)
CPC C12P 7/44 (2013.01) [C12N 9/0069 (2013.01); C12N 9/1022 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12N 15/77 (2013.01); C12Y 113/11001 (2013.01); C12Y 202/01001 (2013.01); C12Y 401/01061 (2013.01); C12Y 401/01063 (2013.01); C12Y 402/0101 (2013.01); C12Y 402/03004 (2013.01)] 5 Claims
 
1. A genetically engineered bacterium utilizing glucose for de novo synthesis of cis, cis-muconic acid, wherein
the genetically engineered bacterium is Corynebacterium glutamicum ATCC13032 recombinantly modified to comprise a cis,cis-muconic acid pathway construction module and an intermediate high-yield module and further having knockouts of pyk, aroE, pcaG/H and CatB genes; wherein
the cis,cis-muconic acid pathway construction module comprises a protocatechuate decarboxylase gene comprising SEQ ID NO: 37, an UbiX-like flavin prenyltransferase gene comprising SEQ ID NO: 68 or SEQ ID NO: 38, a 4-hydroxybenzoate decarboxylase subunit D gene comprising SEQ ID NO: 69 or SEQ ID NO: 39, and a catechol 1,2-dioxygenase gene comprising SEQ ID NO: 76 or SEQ ID NO: 35; and
the intermediate high-yield module comprises a 3-dehydroquinate synthase gene comprising SEQ ID NO: 51, a 3-dehydroquinate dehydratase gene comprising SEQ ID NO: 52, and a transketolase gene comprising SEQ ID NO: 53.