	US 12,344,875 B2
	Sialyltransferases for the production of sialylated oligosaccharides
Joeri Beauprez, Zwijnaarde (BE); Thomas Decoene, Zwijnaarde (BE); Annelies Vercauteren, Zwijnaarde (BE); and Tom Verhaeghe, Zwijnaarde (BE)
Assigned to INBIOSE N.V., Zwijnaarde (BE)
Appl. No. 18/847,017
Filed by INBIOSE N.V., Zwijnaarde (BE)
PCT Filed Mar. 30, 2023, PCT No. PCT/EP2023/058392 § 371(c)(1), (2) Date Sep. 13, 2024, PCT Pub. No. WO2023/187109, PCT Pub. Date Oct. 5, 2023.
Claims priority of application No. 22166399 (EP), filed on Apr. 1, 2022; application No. 501775 (LU), filed on Apr. 1, 2022; and application No. 23151744 (EP), filed on Jan. 16, 2023.
Prior Publication US 2025/0109419 A1, Apr. 3, 2025
Int. Cl. C12P 19/18 (2006.01); C12N 9/10 (2006.01); C12P 19/04 (2006.01)

CPC C12P 19/04 (2013.01) [C12N 9/1081 (2013.01); C12P 19/18 (2013.01); C12Y 204/99 (2013.01)]

17 Claims

1. A method of producing a 3′sialylated oligosaccharide, the method comprising:

contacting a sialyltransferase with a mixture comprising a donor comprising a sialic acid residue and an acceptor selected from an oligosaccharide or a disaccharide under conditions wherein the sialyltransferase catalyzes transfer of the sialic acid residue from the donor to the acceptor, thereby producing the 3′sialylated oligosaccharide,

wherein the sialyltransferase has α-2,3-sialyltransferase activity on the galactose (Gal) residue of lactose and comprises an amino acid sequence that has at least 80.0% amino acid sequence identity to the full-length amino acid sequence of SEQ ID NO: 5,

wherein the contacting occurs in a cell-free system or a cell that is heterologous to the sialyltransferase, and,

optionally, separating the produced 3′sialylated oligosaccharide.