US 12,344,868 B2
ENPP1 polypeptides and methods of using same
Paul Stabach, Woodbridge, CT (US); and Demetrios Braddock, Guilford, CT (US)
Assigned to YALE UNIVERSITY, New Haven, CT (US)
Appl. No. 17/271,018
Filed by YALE UNIVERSITY, New Haven, CT (US)
PCT Filed Aug. 31, 2019, PCT No. PCT/US2019/049243
§ 371(c)(1), (2) Date Feb. 24, 2021,
PCT Pub. No. WO2020/047520, PCT Pub. Date Mar. 5, 2020.
Claims priority of provisional application 62/830,247, filed on Apr. 5, 2019.
Claims priority of provisional application 62/725,607, filed on Aug. 31, 2018.
Prior Publication US 2022/0119782 A1, Apr. 21, 2022
Int. Cl. C12N 9/14 (2006.01); A61K 38/00 (2006.01)
CPC C12N 9/14 (2013.01) [A61K 38/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/30 (2013.01); C12Y 306/01009 (2013.01)] 18 Claims
 
1. An ENPP1 polypeptide fusion comprising an ENPP1 polypeptide fused to a Fc region of an immunoglobulin,
wherein the ENPP1 polypeptide comprises at least one mutation selected from the group consisting of C25N, K27T, V29N, K369N, I371T, P534N, V536T, R545T, P554L, E592N, R741D, and S766N as relating to SEQ ID NO:7.