Biocatalysts and methods for the synthesis of armodafinil
Ee Lui Ang, Singapore (SG); Oscar Alvizo, Fremont, CA (US); Behnaz Behrouzian, Sunnyvale, CA (US); Michael D. Clay, Menlo Park, CA (US); Steven J. Collier, Concord, MA (US); Ellen D. Eberhard, Fallbrook, CA (US); Fu Fan, Singapore (SG); Shiwei Song, Singapore (SG); Derek J. Smith, Singapore (SG); Magnus Widegren, Craigavon (GB); Robert Wilson, Meldreth (GB); Junye Xu, Singapore (SG); and Jun Zhu, Chandler, AZ (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by CODEXIS, INC., Redwood City, CA (US)
Filed on Jun. 6, 2022, as Appl. No. 17/833,788.
Application 14/997,277 is a division of application No. 13/992,138, granted, now 9,267,159, issued on Feb. 23, 2016, previously published as PCT/US2011/063809, filed on Dec. 7, 2011.
Application 17/833,788 is a continuation of application No. 17/184,427, filed on Feb. 24, 2021, granted, now 11,384,343.
Application 17/184,427 is a continuation of application No. 16/995,444, filed on Aug. 17, 2020, granted, now 10,961,517, issued on Mar. 30, 2021.
Application 16/995,444 is a continuation of application No. 16/716,239, filed on Dec. 16, 2019, granted, now 10,781,429, issued on Sep. 22, 2020.
Application 16/716,239 is a continuation of application No. 16/524,468, filed on Jul. 29, 2019, granted, now 10,557,126, issued on Feb. 11, 2020.
Application 16/524,468 is a continuation of application No. 16/113,684, filed on Aug. 27, 2018, granted, now 10,400,223, issued on Sep. 3, 2019.
Application 16/113,684 is a continuation of application No. 15/903,264, filed on Feb. 23, 2018, granted, now 10,087,426, issued on Oct. 2, 2018.
Application 15/903,264 is a continuation of application No. 15/698,319, filed on Sep. 7, 2017, granted, now 9,938,509, issued on Apr. 10, 2018.
Application 15/698,319 is a continuation of application No. 15/352,970, filed on Nov. 16, 2016, granted, now 9,765,306, issued on Sep. 19, 2017.
Application 15/352,970 is a continuation of application No. 15/159,578, filed on May 19, 2016, granted, now 9,528,095, issued on Dec. 27, 2016.
Application 15/159,578 is a continuation of application No. 14/997,277, filed on Jan. 15, 2016, granted, now 9,365,835, issued on Jun. 14, 2016.
Claims priority of provisional application 61/421,123, filed on Dec. 8, 2010.
Prior Publication US 2022/0372452 A1, Nov. 24, 2022
1. A non-naturally occurring polynucleotide encoding a non-naturally occurring polypeptide having cyclohexanone monooxygenase (CHMO) activity, wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to SEQ ID NO: 2, wherein said polypeptide comprises an amino acid substitution at a position corresponding to position 143 in the polypeptide of SEQ ID NO: 2, wherein the amino acid at the position corresponding to position 143 of the polypeptide of SEQ ID NO: 2 is selected from cysteine, glutamate, glycine, histidine, lysine, methionine, proline, glutamine, serine, threonine, and tryptophan.