	US 12,344,859 B2
	Method of introducing target-specific foreign gene
Shodai Komatsu, Tokyo (JP); Kenta Komura, Tokyo (JP); and Yuuji Wakahara, Tokyo (JP)
Assigned to CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo (JP)
Appl. No. 17/600,319
Filed by Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
PCT Filed Apr. 1, 2020, PCT No. PCT/JP2020/014980 § 371(c)(1), (2) Date Sep. 30, 2021, PCT Pub. No. WO2020/204055, PCT Pub. Date Oct. 8, 2020.
Claims priority of application No. 2019-070329 (JP), filed on Apr. 2, 2019.
Prior Publication US 2022/0170047 A1, Jun. 2, 2022
Int. Cl. C12N 5/16 (2006.01); C07K 16/00 (2006.01); C12N 15/85 (2006.01); C12N 15/90 (2006.01); C12P 21/02 (2006.01)

CPC C12N 15/90 (2013.01) [C07K 16/00 (2013.01)]

12 Claims

1. A method for integrating an exogenous DNA encoding a polypeptide of interest into the genome of a Chinese hamster ovary (CHO) cell, wherein the method comprises integrating the exogenous DNA into the first intron of the coiled-coil domain-containing protein 91 (CCDC91) gene of the genome of the CHO cell, wherein the nucleotide sequence of the first intron is SEQ ID NO: 3.