US 12,344,855 B2
Systems for cell control
Stan Wang, Cambridge, MA (US); Suvi Aivio, Cambridge, MA (US); Matthias Wagner, Cambridge, MA (US); Catherine Pilsmaker, Cambridge, MA (US); and Marinna Madrid, Cambridge, MA (US)
Assigned to Cellino Biotech, Inc., Cambridge, MA (US)
Appl. No. 17/291,591
Filed by Cellino Biotech, Inc., Cambridge, MA (US)
PCT Filed Nov. 5, 2019, PCT No. PCT/US2019/059874
§ 371(c)(1), (2) Date May 5, 2021,
PCT Pub. No. WO2020/097083, PCT Pub. Date May 14, 2020.
Claims priority of provisional application 62/928,076, filed on Oct. 30, 2019.
Claims priority of provisional application 62/928,081, filed on Oct. 30, 2019.
Claims priority of provisional application 62/928,078, filed on Oct. 30, 2019.
Claims priority of provisional application 62/841,944, filed on May 2, 2019.
Claims priority of provisional application 62/756,520, filed on Nov. 6, 2018.
Prior Publication US 2021/0403942 A1, Dec. 30, 2021
Int. Cl. C12N 15/85 (2006.01); C12N 5/00 (2006.01); C12N 5/074 (2010.01); C12N 5/079 (2010.01); C12N 9/22 (2006.01); C12N 13/00 (2006.01); C12N 15/11 (2006.01); C12N 15/87 (2006.01); C12N 15/90 (2006.01); G01N 15/1433 (2024.01); G01N 15/1434 (2024.01); G03F 7/00 (2006.01); G03F 7/20 (2006.01); G01N 15/10 (2006.01)
CPC C12N 15/85 (2013.01) [C12N 5/0068 (2013.01); C12N 5/0621 (2013.01); C12N 5/0696 (2013.01); C12N 9/22 (2013.01); C12N 13/00 (2013.01); C12N 15/11 (2013.01); C12N 15/87 (2013.01); C12N 15/907 (2013.01); G01N 15/1433 (2024.01); G01N 15/1434 (2013.01); G03F 7/2006 (2013.01); G03F 7/70025 (2013.01); G03F 7/7055 (2013.01); C12N 2310/20 (2017.05); C12N 2506/08 (2013.01); C12N 2506/45 (2013.01); C12N 2800/80 (2013.01); G01N 2015/1006 (2013.01)] 34 Claims
 
1. A system, the system comprising:
a surface having cells cultured on the surface;
a laser scanning subsystem positioned to emit coherent light towards the surface;
an imaging subsystem positioned to receive light from the surface; and
a computer processor programmed to receive an image of cells from the imaging subsystem and operate the laser scanning subsystem to use the coherent light to selectively remove one or more cells from the surface to manage the cells cultured on the surface, wherein managing the cells comprises at least one of:
controlling local cell density of the cells cultured on the surface;
removing cells that are not properly differentiating;
removing cells based at least in part on predicted functional characteristics; and
removing cells based at least in part on a ratio of cell types of the cells cultured on the surface.