US 12,344,852 B2
Control of phenotype in plants
Sekhar Boddupalli, Davis, CA (US); Andrey Boukharov, Melrose, MA (US); Rio Stamler, Davis, CA (US); Zhongsen Li, Hockessin, DE (US); Arianne Tremblay, Davis, CA (US); Stephen Schauer, Rockville, MD (US); Shiv B. Tiwari, San Jose, CA (US); and John Salmeron, Hillsborough, NC (US)
Assigned to GREENVENUS, LLC, Davis, CA (US)
Filed by GREENVENUS, LLC, Davis, CA (US)
Filed on Dec. 6, 2022, as Appl. No. 18/062,394.
Application 18/062,394 is a division of application No. 16/316,783, granted, now 11,555,197, previously published as PCT/US2017/043650, filed on Jul. 25, 2017.
Claims priority of provisional application 62/467,958, filed on Mar. 7, 2017.
Claims priority of provisional application 62/468,012, filed on Mar. 7, 2017.
Claims priority of provisional application 62/366,402, filed on Jul. 25, 2016.
Prior Publication US 2023/0130408 A1, Apr. 27, 2023
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8217 (2013.01) [C12N 15/8218 (2013.01); C12N 15/825 (2013.01); C12N 15/827 (2013.01); C12N 15/8273 (2013.01); C12N 15/8274 (2013.01); C12N 15/8281 (2013.01); C12N 15/8282 (2013.01); C12N 15/8283 (2013.01); C12N 15/8286 (2013.01)] 16 Claims
 
1. One or more vectors or polynucleotides comprising:
(a) a polynucleotide encoding a VGEvy gene switch, comprising:
(i) a transactivation domain;
(ii) a DNA-binding domain; and
(iii) a ligand-binding domain having the amino acid sequence of SEQ ID NO:2;
(b) a nucleic acid encoding a protein or an RNA that inhibits a fungus; and
(c) a regulatory element operably connected 5′ of (b)
wherein the VGEvy gene switch induces expression of (b) in the presence of a chemical ligand.