US 12,344,848 B2
Modified AOX1 promoter variants
Pinar Calik, Ankara (TR); and Burcu Gunduz Ergun, Ankara (TR)
Assigned to ORTA DOGU TEKNIK UNIVERSITESI, Ankara (TR)
Appl. No. 17/276,168
Filed by ORTA DOGU TEKNIK UNIVERSITESI, Ankara (TR)
PCT Filed Sep. 23, 2019, PCT No. PCT/TR2019/050783
§ 371(c)(1), (2) Date Mar. 15, 2021,
PCT Pub. No. WO2020/068018, PCT Pub. Date Apr. 2, 2020.
Claims priority of application No. 2018/13790 (TR), filed on Sep. 24, 2018.
Prior Publication US 2022/0033832 A1, Feb. 3, 2022
Int. Cl. C12N 15/81 (2006.01)
CPC C12N 15/815 (2013.01) 18 Claims
 
1. A Pichia pastoris alcohol oxidase 1 (AOX1) promoter variant, comprising at least one mutation within nucleotides 1 to 840 of a nucleic acid sequence of a wild-type P. pastoris AOX1 promoter, wherein
the nucleic acid sequence of the wild type P. pastoris AOX1 promoter has the sequence of SEQ ID NO: 1;
the at least one mutation is selected from the group consisting of integration of a transcription factor binding site (TFBS) having the sequence of SEQ ID NO: 6, integration of a TFBS having the sequence of SEQ ID NO: 7, integration of a TFBS having the sequence of SEQ ID NO: 8, integration of a TFBS having the sequence of SEQ ID NO:
9, integration of a TFBS having the sequence of SEQ ID NO: 10 and integration of a TFBS having the sequence of SEQ ID NO: 11; and
the Pichia pastoris AOX1 promoter variant is induced with one or more selected from the group consisting of ethanol and methanol.