US 12,344,847 B2
Cell-associated heterologous food and/or feed enzymes
Aaron Argyros, Lebanon, NH (US); Michelle Oeser, Croydon, NH (US); Erin Wiswall, Danbury, NH (US); Janet Fisher, Enfield, VT (US); Johannes Van Eijk, Longueuil (CA); J. Kevin Kraus, Tenafly, NJ (US); Kevin Wenger, Hanover, NH (US); Brooks Henningsen, Salisbury, NH (US); and Ryan Skinner, South Royalton, VT (US)
Assigned to DANSTAR FERMENT AG, Zug (CH)
Appl. No. 16/493,245
Filed by Lallemand Hungary Liquidity Management LLC, Budapest (HU)
PCT Filed Mar. 13, 2018, PCT No. PCT/IB2018/051670
§ 371(c)(1), (2) Date Sep. 11, 2019,
PCT Pub. No. WO2018/167669, PCT Pub. Date Sep. 20, 2018.
Claims priority of provisional application 62/470,568, filed on Mar. 13, 2017.
Claims priority of provisional application 62/625,751, filed on Feb. 2, 2018.
Prior Publication US 2020/0087672 A1, Mar. 19, 2020
Int. Cl. A23K 10/18 (2016.01); A21D 8/04 (2006.01); A23K 20/189 (2016.01); A23L 33/14 (2016.01); C12N 1/18 (2006.01); C12N 9/16 (2006.01); C12N 9/30 (2006.01); C12N 9/34 (2006.01); C12N 15/81 (2006.01)
CPC C12N 15/81 (2013.01) [A21D 8/047 (2013.01); A23K 10/18 (2016.05); A23K 20/189 (2016.05); A23L 33/14 (2016.08); C12N 1/18 (2013.01); C12N 9/16 (2013.01); C12N 9/242 (2013.01); C12N 9/2428 (2013.01); C12Y 302/01133 (2013.01)] 15 Claims
 
1. A process for making a food or a feed product, said process comprising including a recombinant yeast host cell or an additive comprising the recombinant yeast host cell in the food or the feed product, wherein the recombinant yeast host cell has a heterologous nucleic acid molecule encoding a cell-associated heterologous maltogenic alpha-amylase, wherein the maltogenic alpha-amylase has at least 80% identity to the amino acid sequence of SEQ ID NO: 1, 51, 65 or 108, wherein the heterologous nucleic acid molecule is operatively associated with a promoter allowing expression of the heterologous nucleic acid molecule during propagation under aerobic conditions.