	US 12,344,824 B2
	Culture chamber and culture method
Yoko Ejiri, Tsukuba (JP); Satoru Ayano, Tsukuba (JP); Naoto Fukuhara, Tsukuba (JP); Hideki Taniguchi, Yokohama (JP); and Takanori Takebe, Yokohama (JP)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Filed by CORNING INCORPORATED, Corning, NY (US); and Public University Corporation Yokohama City University, Yokohama (JP)
Filed on Nov. 28, 2023, as Appl. No. 18/521,821.
Application 18/521,821 is a division of application No. 17/957,743, filed on Sep. 30, 2022, granted, now 11,866,682.
Application 17/957,743 is a division of application No. 16/668,701, filed on Oct. 30, 2019, granted, now 11,473,046, issued on Oct. 18, 2022.
Application 16/668,701 is a division of application No. 14/896,251, granted, now 10,494,593, issued on Dec. 3, 2019, previously published as PCT/JP2014/002993, filed on Jun. 5, 2014.
Claims priority of application No. 2013-120915 (JP), filed on Jun. 7, 2013.
Prior Publication US 2024/0093134 A1, Mar. 21, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/32 (2006.01); C12M 1/24 (2006.01)

CPC C12M 23/08 (2013.01) [C12M 23/12 (2013.01)]

15 Claims

1. A culture chamber comprising:

a plurality of recesses each configured to receive a cell, each recess of said plurality of recesses formed of a bottom portion and an opening portion, wherein:

the bottom portion of each recess having a depth D1 and comprises a hemispherical shape,

the opening portion of each recess having a depth D2 is defined by a wall that surrounds an area from a boundary between the opening portion and the bottom portion to an end of each of the recesses, wherein the total depth D of each recess equals D1+D2,

an equivalent diameter of the boundary is from 50 μm to 1 mm and D is from 0.6 or more times to 3 or less times of the equivalent diameter,

wherein an area between two adjacent recesses is flat and a distance between the two recesses is in a range from 5 μm to 50 μm, and

wherein the surface of each recess comprises a hydrophilic polymer.