US 12,344,769 B2
Method for extracting gelatin
Mingzhu Wang, Beijing (CN); Yu Zhang, Beijing (CN); and Yazhen Wang, Beijing (CN)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Appl. No. 17/439,859
Filed by Novozymes A/S, Bagsvaerd (DK)
PCT Filed Apr. 7, 2020, PCT No. PCT/CN2020/083470
§ 371(c)(1), (2) Date Sep. 16, 2021,
PCT Pub. No. WO2020/207370, PCT Pub. Date Oct. 15, 2020.
Prior Publication US 2022/0186079 A1, Jun. 16, 2022
Int. Cl. C12P 19/00 (2006.01); C09H 3/00 (2006.01); C09H 7/00 (2006.01); C12P 21/06 (2006.01)
CPC C09H 3/00 (2013.01) [C09H 7/00 (2013.01); C12P 21/06 (2013.01)] 9 Claims
 
1. A method for extracting gelatin, comprising:
(a) demineralizing a collagen containing material to produce ossein;
(b) grinding the ossein to particles that can pass 10 meshes sieves;
(c) incubating the ground ossein in the presence of an effective amount of a protease, and
(d) extracting the gelatin from the ground ossein, wherein the protease is a metalloprotease belonging to family M35 and comprises of an amino acid sequence having at least 85% identity to amino acids 1-177 of the amino acid sequence SEQ ID NO: 1.