	US 12,344,714 B2
	Esterases and uses thereof
Alain Marty, Toulouse (FR); and Vincent Tournier, Toulouse (FR)
Assigned to CARBIOS, Clermont-Ferrand (FR)
Appl. No. 17/625,783
Filed by CARBIOS, Clermont-Ferrand (FR)
PCT Filed Jul. 10, 2020, PCT No. PCT/EP2020/069499 § 371(c)(1), (2) Date Jan. 10, 2022, PCT Pub. No. WO2021/005198, PCT Pub. Date Jan. 14, 2021.
Claims priority of application No. 19185789 (EP), filed on Jul. 11, 2019.
Prior Publication US 2022/0251327 A1, Aug. 11, 2022
Int. Cl. C08J 11/10 (2006.01); C11D 3/386 (2006.01); C12N 1/21 (2006.01); C12N 9/18 (2006.01)

CPC C08J 11/105 (2013.01) [C11D 3/38636 (2013.01); C12N 9/18 (2013.01); C12Y 301/01074 (2013.01)]

33 Claims

1. An esterase which (i) has at least 75% identity to the full length amino acid sequence set forth in SEQ ID NO: 1, (ii) comprises at least the combination of amino acid substitutions selected from the group consisting of L210T/A/R/W/H+V172I and L210T/A/R/W/H+V172, wherein the position is numbered by reference to the amino acid sequence set forth in SEQ ID NO: 1, and (iii) has an increased degrading activity and/or an increased thermostability as compared to the esterase of SEQ ID NO: 1.