US 12,343,724 B2
Detection chip, method for using detection chip, and detection apparatus
Lijiao Hu, Beijing (CN); Haochen Cui, Beijing (CN); Xiaohe Shen, Beijing (CN); Chungen Yuan, Beijing (CN); Tao Hu, Beijing (CN); Jing Li, Beijing (CN); and Weiqiong Gan, Beijing (CN)
Assigned to BEIJING BOE HEALTH TECHNOLOGY CO., LTD., Beijing (CN); and BOE TECHNOLOGY GROUP CO., LTD., Beijing (CN)
Appl. No. 17/614,601
Filed by BEIJING BOE HEALTH TECHNOLOGY CO., LTD., Beijing (CN); and BOE TECHNOLOGY GROUP CO., LTD., Beijing (CN)
PCT Filed Feb. 1, 2021, PCT No. PCT/CN2021/074637
§ 371(c)(1), (2) Date Nov. 29, 2021,
PCT Pub. No. WO2021/164531, PCT Pub. Date Aug. 26, 2021.
Claims priority of application No. 202010104107.6 (CN), filed on Feb. 20, 2020.
Prior Publication US 2022/0226824 A1, Jul. 21, 2022
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502753 (2013.01) [B01L 3/502707 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0861 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A detection chip, comprising a chip substrate,
wherein the chip substrate comprises a fluid channel and a plurality of liquid cells, and the fluid channel is arranged on a surface of the chip substrate and comprises a main path and a plurality of branch paths,
the plurality of branch paths are respectively communicated to the plurality of liquid cells, the plurality of branch paths are all communicated to the main path, communication points between the plurality of branch paths and the main path are different, and an included angle between at least one of the plurality of branch paths and the main path is an acute angle,
the plurality of branch paths are configured to allow liquid in the plurality of branch paths to be capable of merging into the main path in a same direction and flowing in the main path long a merging direction under action of inertia,
wherein the fluid channel further comprises an extraction region, and the extraction region is communicated to the main path;
the detection chip further comprises a sealing film,
wherein the sealing film covers the surface of the chip substrate having the fluid channel,
wherein the fluid channel further comprises a plurality of flow paths and a plurality of film valve parts,
the chip substrate further comprises a reaction cell and a waste liquid cell, the reaction cell is configured to contain liquid that needs to be subjected to an amplification reaction, and the waste liquid cell is configured to contain waste liquid generated in the extraction region during reaction process, and the reaction cell and the waste liquid cell are respectively communicated to the extraction region through the plurality of flow paths,
the plurality of film valve parts are respectively in the plurality of flow paths, and a film valve part of the plurality of film valve parts is configured to allow a portion of the sealing film covering the film valve part to approach and separate, so as to correspondingly close and open a flow path,
wherein the reaction cell comprises a porous structure, the porous structure comprises a plurality of pore-shaped parts, and the plurality of pore-shaped parts are configured to contain same or different amplification primers,
wherein the porous structure further comprises a connecting channel and a plurality of connecting byroads, the plurality of connecting byroads are all communicated to the connecting channel, and an extending direction of the connecting byroads is perpendicular to an extending direction of the connecting channel, and
the plurality of pore-shaped parts are respectively communicated to the plurality of connecting byroads, respectively, and the plurality of pore-shaped parts are arranged in a row along a direction parallel to the extending direction of the connecting channel.