US 12,343,723 B2
Methods and compositions for surface functionalization of optical semiconductor-integrated biochips
Andrea Cuppoletti, Livermore, CA (US); Arjang Hassibi, Santa Clara, CA (US); Lei Pei, San Jose, CA (US); Yang Liu, San Jose, CA (US); Kshama Jirage, Palo Alto, CA (US); and Arun Manickam, San Jose, CA (US)
Assigned to InSilixa, Inc., Santa Clara, CA (US)
Filed by InSilixa, Inc., Santa Clara, CA (US)
Filed on Oct. 24, 2023, as Appl. No. 18/383,385.
Application 18/383,385 is a continuation of application No. 17/544,776, filed on Dec. 7, 2021, granted, now 11,833,503.
Prior Publication US 2024/0335832 A1, Oct. 10, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); B01L 7/00 (2006.01); C07F 7/08 (2006.01); C23C 16/02 (2006.01); H10F 39/18 (2025.01)
CPC B01L 3/502715 (2013.01) [B01L 3/502707 (2013.01); B01L 3/502738 (2013.01); B01L 7/52 (2013.01); C07F 7/089 (2013.01); C23C 16/0272 (2013.01); H10F 39/182 (2025.01); B01L 2200/0689 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/06 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for immobilizing a nucleic acid construct to a surface of a substrate, the method comprising:
contacting a silane on the substrate with the nucleic acid construct and forming a 1,2,3-triazole via a strain-promoted alkyne-azide cycloaddition (SPAAC), thereby immobilizing the nucleic acid construct on the surface of the substrate,
wherein the substrate comprises an integrated circuit.