US 12,343,722 B2
Reagent cartridges for in-vitro devices
Sz-Chin Lin, San Jose, CA (US); Jian Gong, Danville, CA (US); Yiwen Ouyang, San Jose, CA (US); and Yan-You Lin, Fremont, CA (US)
Assigned to MGI Holdings Co., Limited, Shenzhen (CN)
Appl. No. 17/631,086
Filed by MGI Holdings Co., Limited, Shenzhen (CN)
PCT Filed Jul. 28, 2020, PCT No. PCT/CN2020/105030
§ 371(c)(1), (2) Date Jan. 28, 2022,
PCT Pub. No. WO2021/018111, PCT Pub. Date Feb. 4, 2021.
Claims priority of provisional application 62/879,990, filed on Jul. 29, 2019.
Prior Publication US 2022/0250062 A1, Aug. 11, 2022
Int. Cl. B01L 3/00 (2006.01); B01L 9/00 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/527 (2013.01); B01L 9/54 (2013.01); B01L 2200/027 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0829 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0683 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method of transferring reagents to a microfluidic cartridge comprising: positioning a reagent cartridge over a microfluidic device, wherein:
the reagent cartridge comprises: a substrate comprising:
one or more blisters, wherein each blister comprises a fluid reservoir configured to hold a volume of fluid; and
one or more dispensing tips, each dispensing tip comprising a pathway that is fluidly coupled to a blister, wherein a fluid is configured to be displaced from or loaded into the blister via the dispensing tip; and
one or more deformable seals fixed to the substrate and overlaid on the one or more blisters, such that the one or more deformable seals are configured to seal the volumes of fluid within the one or more blisters; and
the microfluidic device comprises a first inlet opening that is fluidly coupled to a first reservoir of the microfluidic device;
wherein positioning the reagent cartridge comprises aligning a first dispensing tip of the one or more dispensing tips with the first inlet opening such that the first inlet opening is configured to receive a first fluid from a first blister fluidly coupled to the first dispensing tip; and
displacing one or more of the deformable seals, such that the one or more deformable seals are configured to cause a first volume of the first fluid to be displaced from the first blister into the first reservoir via the first inlet opening.