US 12,343,721 B2
Microfluidic chip, microfluidic device, associated uses and methods
Luis José Fernández Ledesma, Saragossa (ES); Rosa Maria Monge Prieto, Saragossa (ES); Jose Luis Calavia Calvo, Saragossa (ES); Jorge Santolaria Mazo, Saragossa (ES); Javier Orús Pontaque, Saragossa (ES); Carlos Peribáñez Subirón, Saragossa (ES); José Manuel Rodriguez Fortún, Saragossa (ES); and Ignacio Ochoa Garrido, Madrid (ES)
Assigned to UNIVERSIDAD DE ZARAGOZA, Saragossa (ES); INSTITUTO TECNOLÓGICO DE ARAGÓN, Saragossa (ES); and CENTRO DE INVESTIGACIÓN BIOMÉDICA EN RED, Madrid (ES)
Appl. No. 15/771,161
Filed by UNIVERSIDAD DE ZARAGOZA, Saragossa (ES); INSTITUTO TECNOLÓGICO DE ARAGÓN, Saragossa (ES); and CENTRO DE INVESTIGACIÓN BIOMÉDICA EN RED, Madrid (ES)
PCT Filed Oct. 14, 2016, PCT No. PCT/ES2016/070726
§ 371(c)(1), (2) Date Apr. 26, 2018,
PCT Pub. No. WO2017/072379, PCT Pub. Date May 4, 2017.
Claims priority of application No. 201531539 (ES), filed on Oct. 27, 2015.
Prior Publication US 2018/0333721 A1, Nov. 22, 2018
Int. Cl. B01L 3/00 (2006.01); B01L 9/00 (2006.01); B32B 3/30 (2006.01); B32B 25/08 (2006.01); B32B 37/16 (2006.01); B32B 37/18 (2006.01); B32B 38/00 (2006.01); F16K 99/00 (2006.01)
CPC B01L 3/502707 (2013.01) [B01L 3/00 (2013.01); B01L 3/502715 (2013.01); B01L 3/502738 (2013.01); B01L 9/50 (2013.01); B32B 3/30 (2013.01); B32B 25/08 (2013.01); B32B 37/16 (2013.01); B32B 37/182 (2013.01); B32B 38/0008 (2013.01); F16K 99/0015 (2013.01); F16K 99/0048 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/12 (2013.01); B01L 2300/043 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0484 (2013.01); B01L 2400/06 (2013.01); B01L 2400/0638 (2013.01); B01L 2400/0655 (2013.01); F16K 2099/0084 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for the fabrication of a microfluidic chip of the type that comprises:
a deformable sealing layer, wherein said layer comprises a flexible, elastic, and insulating material;
a structure formed by an arrangement of micro-structured layers comprising a thermoplastic material, wherein said structure comprises:
one or several micro-chambers;
one or several microfluidic channels for the circulation of fluid to and from the micro-chambers, and;
one or several fluidic inlets and outlets;
and a base substrate on which the structure of the micro-structured thermoplastic layers is arranged;
said method characterized in that it comprises the following steps:
a) applying a plasma cleaning treatment to the structure of micro-structured thermoplastic layers and to the deformable sealing layer;
b) after step a), applying (3-Aminopropyl triethoxysilane) (APTES) to the structure of micro-structured thermoplastic layers and to the deformable sealing layer, wherein APTES comprises amine (—NH2) and hydroxyl (—OH) free radicals;
c) after step b), arranging the deformable sealing layer on the structure of micro-structured thermoplastic layers;
d) after step c), applying a sealing temperature between 70 and 100° C. and a sealing pressure between 0.1 and 5 bar to the micro-structured thermoplastic layers and the deformable sealing layer for a sealing time between 5 and 45 minutes to seal said micro-structured thermoplastic layers to the deformable layer.