US 12,343,433 B2
Microencapsulation method for improving stability of anthocyanin, product therefrom and use thereof
Jingren He, Hubei (CN); Lan Zhou, Hubei (CN); Rui Zhang, Hubei (CN); Ying Mao, Hubei (CN); Ning Yang, Hubei (CN); Shuyi Li, Hubei (CN); Yi He, Hubei (CN); and Zhenzhou Zhu, Hubei (CN)
Assigned to WUHAN POLYTECHNIC UNIVERSITY, Wuhan (CN)
Filed by WUHAN POLYTECHNIC UNIVERSITY, Hubei (CN)
Filed on Oct. 17, 2021, as Appl. No. 17/503,351.
Application 17/503,351 is a continuation of application No. PCT/CN2020/084963, filed on Apr. 15, 2020.
Claims priority of application No. 201910309622.5 (CN), filed on Apr. 17, 2019.
Prior Publication US 2022/0071914 A1, Mar. 10, 2022
Int. Cl. A61K 9/50 (2006.01); A23L 5/43 (2016.01); A23L 29/00 (2016.01); A23L 29/256 (2016.01); A23P 10/35 (2016.01); A61K 47/22 (2006.01); B01D 15/20 (2006.01); B01D 15/42 (2006.01); B01D 61/02 (2006.01); B01D 61/04 (2006.01); B01D 61/14 (2006.01); B01D 61/16 (2006.01); B01D 61/58 (2006.01); B01D 69/02 (2006.01)
CPC A61K 9/5089 (2013.01) [A23L 5/43 (2016.08); A23L 29/015 (2016.08); A23L 29/035 (2016.08); A23L 29/256 (2016.08); A23P 10/35 (2016.08); A61K 9/5036 (2013.01); A61K 47/22 (2013.01); B01D 15/203 (2013.01); B01D 15/426 (2013.01); B01D 61/025 (2013.01); B01D 61/027 (2013.01); B01D 61/04 (2013.01); B01D 61/145 (2013.01); B01D 61/147 (2013.01); B01D 61/149 (2022.08); B01D 61/16 (2013.01); B01D 61/58 (2013.01); B01D 69/02 (2013.01); A23V 2002/00 (2013.01); B01D 2311/04 (2013.01); B01D 2311/06 (2013.01); B01D 2311/2626 (2013.01); B01D 2311/2649 (2013.01); B01D 2311/2676 (2013.01)] 8 Claims
 
1. A microencapsulation method for improving stability of anthocyanins, comprising the following steps:
S1, taking sodium alginate as a wall material, respectively preparing sodium alginate, calcium carbonate and water according to a weight ratio of sodium alginate to calcium carbonate to water of (2-4):1:(15-25), and then adding the sodium alginate and the calcium carbonate into water to swell for 1-2 h to obtain a wall material gel system;
S2, taking anthocyanins as a core material, and fully and uniformly mixing the wall material gel system with an anthocyanin solution for later use at a weight ratio of the sodium alginate to the anthocyanins of (12-20):1 to obtain a water phase;
S3, mixing sorbitan monooleate with vegetable oil at a volume ratio of (1-2):1 to obtain an oil phase, mixing the water phase with the oil phase at a volume ratio of (3-5):1, and magnetically stirring the mixture for emulsifying to obtain a W/O emulsion; and
S4, adjusting the pH of the W/O emulsion to be acidic, mixing the W/O emulsion with a buffer solution at a volume ratio of 1:(3-5), standing for 1-2 h, and separating the oil phase from the water phase to obtain a liquid anthocyanin microcapsule.