US 12,343,408 B2
AAV-mediated subcellular targeting of heterologous rhodopsins in retinal ganglion cells
Zhuo-Hua Pan, Troy, MI (US)
Assigned to Wayne State University, Detroit, MI (US)
Filed by Wayne State University, Detroit, MI (US)
Filed on Mar. 8, 2021, as Appl. No. 17/195,288.
Application 17/195,288 is a continuation of application No. 15/978,290, filed on May 14, 2018, abandoned.
Application 15/978,290 is a continuation of application No. 15/236,152, filed on Aug. 12, 2016, granted, now 9,968,689, issued on May 15, 2018.
Application 15/236,152 is a continuation of application No. 13/696,252, granted, now 9,453,241, issued on Sep. 27, 2016, previously published as PCT/US2011/035266, filed on May 4, 2011.
Claims priority of provisional application 61/331,125, filed on May 4, 2010.
Prior Publication US 2022/0040326 A1, Feb. 10, 2022
Int. Cl. A61K 48/00 (2006.01); A61K 38/00 (2006.01); A61P 27/02 (2006.01); C07K 14/47 (2006.01); C07K 14/705 (2006.01); C12N 15/861 (2006.01)
CPC A61K 48/005 (2013.01) [C07K 14/4702 (2013.01); C07K 14/705 (2013.01); C12N 15/8616 (2013.01); A61K 38/00 (2013.01); A61P 27/02 (2018.01); C07K 2319/01 (2013.01); C12N 2750/14143 (2013.01)] 18 Claims
 
1. A polynucleotide molecule comprising a nucleic acid sequence encoding a rhodopsin for expression in subcellular regions of a retinal neuron, comprising:
(a) a first nucleotide sequence encoding a light-gated channel rhodopsin or a light-driven ion pump rhodopsin;
(b) linked in frame to (a), a second nucleotide sequence encoding a peptide or polypeptide sorting motif, wherein the second nucleotide sequence is selected from the group consisting of:
(1) a nucleotide sequence encoding nicotinic acetylcholine receptor a7 subunit (nAchR) comprising SEQ ID NO:9,
(2) a nucleotide sequence encoding voltage-gated potassium channel 4.2 (Kv4.2) comprising SEQ ID NO:11,
(3) a nucleotide sequence encoding telencephalin (TLCN) comprising SEQ ID NO:13, and
(4) a nucleotide sequence encoding AMPA receptor GluR1 subunit comprising SEQ ID NO:15;
(c) operatively linked to (a) and (b), a promoter sequence; and
(d) a polyadenylation sequence.