| CPC A61K 31/497 (2013.01) [A61K 31/519 (2013.01); A61P 35/00 (2018.01)] | 6 Claims |

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1. A method of treating cancer comprising administering to a subject in need thereof comprising (3S,4S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio) pyrazin-2-yl)-3-methyl-2-oxa-8-azaspiro [4.5]decan-4-amine, or a pharmaceutically acceptable salt thereof, in combination with a second therapeutic agent,
wherein the second therapeutic agent is the CDK4/6 inhibitor 7-cyclopentyl-N,N-dimethyl-2-((5-(piperazin-1-yl) pyridin-2-yl)amino)-7H-pyrrolo [2,3-d]pyrimidine-6-carboxamide, or a pharmaceutically acceptable salt thereof;
wherein the cancer is selected from the group consisting of esophageal or head and neck squamous cell carcinoma; colorectal or non-small cell lung cancer; and renal cell carcinoma; and
wherein the amount of (3S,4S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio) pyrazin-2-y)-3-methyl-2-oxa-8-azaspiro [4.5]decan-4-amine, or a pharmaceutically acceptable salt thereof, and the amount of the second therapeutic agent administered to the subject in need thereof are effective to treat the cancer.
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