US 12,342,838 B2
Method of reducing the self-heating propensity of biomass
Michael Benjamin Johnson, Columbia, MD (US); and Shannon Elizabeth Ethier Resop, Columbia, MD (US)
Assigned to DSM IP ASSETS B.V., Heerlen (NL); and EVONIK OPERATIONS GMBH, Essen (DE)
Appl. No. 17/250,625
Filed by DSM IP ASSETS B.V., Heerlen (NL); and Evonik Operations GmbH, Essen (DE)
PCT Filed Aug. 9, 2019, PCT No. PCT/US2019/045838
§ 371(c)(1), (2) Date Feb. 12, 2021,
PCT Pub. No. WO2020/036814, PCT Pub. Date Feb. 20, 2020.
Claims priority of provisional application 62/718,549, filed on Aug. 14, 2018.
Claims priority of provisional application 62/876,076, filed on Jul. 19, 2019.
Prior Publication US 2021/0321640 A1, Oct. 21, 2021
Int. Cl. A23K 20/158 (2016.01); A23K 10/16 (2016.01); A23K 20/163 (2016.01); A23K 30/20 (2016.01); C12N 1/12 (2006.01); C12P 7/6427 (2022.01); C12P 7/6432 (2022.01); C12P 7/6434 (2022.01); C12P 7/6472 (2022.01)
CPC A23K 20/158 (2016.05) [A23K 10/16 (2016.05); A23K 20/163 (2016.05); A23K 30/20 (2016.05); C12N 1/12 (2013.01); C12P 7/6427 (2013.01); C12P 7/6432 (2022.01); C12P 7/6434 (2022.01); C12P 7/6472 (2013.01)] 9 Claims
 
1. A PUFA-containing composition comprising:
a fermented biomass of cells comprising at least 20 wt % of at least one type of polyunsaturated fatty acid (PUFA) having at least 20 carbon atoms and at least three double bonds, wherein
the PUFA-containing composition has a reduced self-heating propensity sufficient to cause a temperature of the PUFA-containing composition to not spontaneously be increased to 180° C. or more in an oven when the PUFA-containing composition is placed in a 100 mm sample cube and is heated in the oven at 120° C. for 24 hours,
wherein the reduced self-heating propensity is obtained by:
(i) limiting fermentation of the biomass to less than 6 days,
(ii) omitting a pasteurization step after fermentation,
(iii) conducting a drum drying step instead of a lyophilzation step,
(iv) adding at least one type of natural antioxidant and at least one type of synthetic antioxidant to a fermentation broth at the end of fermentation, or
(v) including at least 50 g/L sugar to a fermentation broth at the end of fermentation.