	US 12,019,083 B2
	Compound distribution in microfluidic devices
Josiah Sliz, Boston, MA (US); Daniel Levner, Brookline, MA (US); Brian Zuckerman, Cambridge, MA (US); Norman Wen, West Roxbury, MA (US); Jonathan Rubins, Cambridge, MA (US); Tanvi Shroff, Cambridge, MA (US); Christopher David Hinojosa, Malden, MA (US); Grace Ahn, Medford, MA (US); Victor Antontsev, Somerville, MA (US); Jefferson Puerta, Malden, MA (US); David Conegliano, Boston, MA (US); and S. Jordan Kerns, Reading, MA (US)
Assigned to EMULATE, INC., Boston, MA (US)
Filed by EMULATE, Inc., Boston, MA (US)
Filed on Jun. 7, 2022, as Appl. No. 17/834,165.
Application 17/834,165 is a continuation of application No. 17/538,518, filed on Nov. 30, 2021.
Application 17/538,518 is a continuation of application No. PCT/US2020/039830, filed on Jun. 26, 2020.
Claims priority of provisional application 62/867,543, filed on Jun. 27, 2019.
Prior Publication US 2022/0334139 A1, Oct. 20, 2022
Int. Cl. G01N 35/00 (2006.01); B01L 3/00 (2006.01); C12Q 3/00 (2006.01); G01N 15/06 (2006.01); G01N 33/50 (2006.01)

CPC G01N 35/00584 (2013.01) [B01L 3/502761 (2013.01); C12Q 3/00 (2013.01); G01N 15/06 (2013.01); G01N 33/5008 (2013.01); B01L 3/502707 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/148 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/069 (2013.01); B01L 2300/10 (2013.01); B01L 2400/0406 (2013.01); G01N 2035/00544 (2013.01); G01N 2035/0097 (2013.01)]

9 Claims

1. A method of determining compound distribution in a system, comprising:

a) providing a system and an experimental protocol for said system comprising one or more biological elements; wherein said one or more biological elements are contacted by a compound;

b) modifying said experimental protocol by excluding at least one of said one or more biological elements;

c) performing said modified experimental protocol; and

d) determining the distribution of said compound in said system using by measuring the concentration of said compound in said system.