US 12,019,067 B2
Interferometry based systems to detect small mass changes of an object in a solution
Kayvan Niazi, Agoura Hills, CA (US); and Krsto Sbutega, Redondo Beach, CA (US)
Assigned to NantBio, Inc., Culver City, CA (US)
Filed by NantBio, Inc., Culver City, CA (US)
Filed on May 20, 2021, as Appl. No. 17/325,448.
Claims priority of provisional application 63/028,183, filed on May 21, 2020.
Claims priority of provisional application 63/028,264, filed on May 21, 2020.
Prior Publication US 2021/0364496 A1, Nov. 25, 2021
Int. Cl. G01N 33/00 (2006.01); G01G 9/00 (2006.01); G01N 33/50 (2006.01)
CPC G01N 33/5011 (2013.01) [G01G 9/00 (2013.01); G01N 2500/10 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A computer-implemented method of using interferometry to detect mass changes of objects in a solution comprising:
obtaining a time series of images comprising one or more objects using interferometry;
performing background correction on the images of the time series, wherein the background correction comprises, for each image:
classifying pixels of the image as background pixels or object pixels;
fitting only the background pixels of the image to a function to generate a background fitted function; and
subtracting the background fitted function from the image to generate a background corrected image;
performing segmentation on the background corrected images of the time series to resolve boundaries of the one or more objects;
performing motion tracking on the one or more objects of the segmented background corrected images to track changes in position of the one or more objects;
determining respective masses of the one or more motion tracked objects; and
determining, for each image in the time series an aggregate mass based on the respective masses to determine whether the aggregate mass of the one or more motion tracked objects is increasing or decreasing.