	US 12,018,310 B2
	Methods and microorganisms for making 1,4-butanediol and derivatives thereof from C1 carbons
Xinhua Zhao, Dublin, CA (US); Tina Huynh, Oakland, CA (US); Jeffrey Orth, Alameda, CA (US); Lily Yuin Chao, San Francisco, CA (US); and James Kealey, Sebastopol, CA (US)
Assigned to PRECIGEN, INC., Germantown, MD (US)
Filed by PRECIGEN, INC., Germantown, MD (US)
Filed on Sep. 13, 2021, as Appl. No. 17/447,568.
Application 17/447,568 is a division of application No. 16/492,411, granted, now 11,155,837, previously published as PCT/US2018/022204, filed on Mar. 13, 2018.
Claims priority of provisional application 62/470,953, filed on Mar. 14, 2017.
Prior Publication US 2022/0064677 A1, Mar. 3, 2022
Int. Cl. C12P 7/04 (2006.01); C12N 1/20 (2006.01); C12N 15/74 (2006.01)

CPC C12P 7/04 (2013.01) [C12N 1/20 (2013.01); C12N 15/74 (2013.01)]

20 Claims

1. A genetically modified methanotroph capable of converting a single carbon-containing hydrocarbon molecule to 1,4-butanediol, the genetically modified methanotroph comprising heterologous genes encoding:

(a) an α-ketoglutarate decarboxylase comprising an amino acid sequence that is at least 90% identical to any one of SEQ ID NOs: 1, 3, 5, and 7;

(b) a 4-hydroxybutyrate dehydrogenase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 11 or 13;

(c) a 4-hydroxybutyrate CoA transferase comprising an amino acid sequence that is at least 90% identical to any one of SEQ ID NOs: 15, 17, and 19;

(d) an aldehyde dehydrogenase comprising an amino acid sequence that is at least 90% identical to any one of SEQ ID NOs: 21, 23, 25, 27, 29, 31, 33, and 35; and

(e) an alcohol dehydrogenase comprising an amino acid sequence that is at least 90% identical to any one of SEQ ID NOs: 21, 23, 25, 27, 29, 31, 33, and 35.