	US 12,018,307 B2
	Process for producing a glucuronide and genetically modified microorganisms useful in this process
Jixun Zhan, North Logan, UT (US)
Assigned to UTAH STATE UNIVERSITY, North Logan, UT (US)
Filed by Jixun Zhan, North Logan, UT (US)
Filed on Aug. 26, 2022, as Appl. No. 17/822,733.
Claims priority of provisional application 63/273,136, filed on Oct. 28, 2021.
Prior Publication US 2023/0142611 A1, May 11, 2023
Int. Cl. C12P 19/60 (2006.01); A61K 31/05 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 15/62 (2006.01); C12N 15/63 (2006.01)

CPC C12P 19/60 (2013.01) [A61K 31/05 (2013.01); C12N 9/0006 (2013.01); C12N 9/1051 (2013.01); C12N 9/1241 (2013.01); C12N 15/62 (2013.01); C12N 15/63 (2013.01); C12Y 101/01022 (2013.01); C12Y 204/01017 (2013.01); C12Y 207/07009 (2013.01)]

11 Claims

1. A process for producing a glucuronide from UDP-glucuronic acid and a phenolic compound that comprises a hydroxyl or a carboxyl group, wherein said glucuronide comprises a glucuronic acid moiety bound to the hydroxyl or the carboxyl group of said phenolic compound, wherein said process comprises the steps of:

(i) culturing a genetically modified microorganism in a medium that comprises the phenolic compound, wherein the genetically modified microorganism expresses a UDP-glucuronyltransferase (UGT) that comprises the amino acid sequence of SEQ ID NO:7 or an amino acid sequence at least 95% identical to SEQ ID NO:7, under conditions allowing the genetically modified microorganism to produce the glucuronide; and

(ii) optionally recovering the glucuronide from the medium.