US 12,339,282 B2
Coronavirus antibody detection kit based on magnetic particle chemiluminescence
Chunlong Liu, Binhai New Area Tianjin (CN); Zhou Zhang, Binhai New Area Tianjin (CN); Shaohua Ma, Binhai New Area Tianjin (CN); Jiashun Wang, Binhai New Area Tianjin (CN); Yan Su, Binhai New Area Tianjin (CN); and Zeqi Zhou, Binhai New Area Tianjin (CN)
Assigned to Dynamiker Biotechnology (Tianjin) Co., Ltd., Binhai New Area Tianjin (CN)
Appl. No. 17/436,791
Filed by Dynamiker Biotechnology (Tianjin) Co, Ltd., Binhai New Area Tianjin (CN)
PCT Filed Mar. 24, 2021, PCT No. PCT/CN2021/082727
§ 371(c)(1), (2) Date Sep. 7, 2021,
PCT Pub. No. WO2022/121151, PCT Pub. Date Jun. 16, 2022.
Claims priority of application No. 202011432236.4 (CN), filed on Dec. 10, 2020.
Prior Publication US 2023/0296601 A1, Sep. 21, 2023
Int. Cl. G01N 33/569 (2006.01); G01N 21/76 (2006.01); G01N 33/532 (2006.01); G01N 33/543 (2006.01); G01N 33/563 (2006.01)
CPC G01N 33/56983 (2013.01) [G01N 21/76 (2013.01); G01N 33/532 (2013.01); G01N 33/54326 (2013.01); G01N 33/563 (2013.01); G01N 2469/20 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A novel coronavirus antibody detection kit based on magnetic particle chemiluminescence, comprising:
streptavidin magnetic particles, biotin-labeled novel coronavirus antigens, an acridine sulfonamide-labeled secondary antibody, a sample diluent and a quality control material, wherein the biotin-labeled novel coronavirus antigens include a recombinant nucleocapsid protein and a recombinant spike protein S1;
wherein, in the recombinant nucleocapsid protein, oligoproline residues are used to connect dominant epitopes of an original nucleocapsid protein, and oligolysine residues are used to form a C-terminus;
in the recombinant spike protein S1, oligoproline residues are used to connect dominant epitopes of an original spike protein S1, and oligolysine residues are used to form a C-terminus;
the recombinant nucleocapsid protein includes the amino acid sequence as shown in SEQ ID NO: 1; and
the recombinant spike protein S1 includes the amino acid sequence as shown in SEQ ID NO: 3.