	US 12,339,233 B2
	Optical detection of a substance in fluid
Jacobus Hermanus Maria Neijzen, Heeze (NL); Jeroen Hans Nieuwenhuis, Waalre (NL); Arie Rombertus Van Doorn, Zaltbommel (NL); and Matthias Irmscher, Eindhoven (NL)
Assigned to Siemens Healthineers Nederland B.V., The Hague (NL)
Appl. No. 16/065,458
Filed by SIEMENS HEALTHINEERS NEDERLAND B.V., The Hague (NL)
PCT Filed Dec. 20, 2016, PCT No. PCT/EP2016/081819 § 371(c)(1), (2) Date Jun. 22, 2018, PCT Pub. No. WO2017/108726, PCT Pub. Date Jun. 29, 2017.
Claims priority of application No. 15202672 (EP), filed on Dec. 24, 2015.
Prior Publication US 2019/0025210 A1, Jan. 24, 2019
Int. Cl. G01N 21/64 (2006.01); G01N 21/552 (2014.01); G01N 21/76 (2006.01); G01N 33/543 (2006.01)

CPC G01N 21/648 (2013.01) [G01N 21/552 (2013.01); G01N 21/76 (2013.01); G01N 33/54326 (2013.01); G01N 33/54373 (2013.01)]

28 Claims

1. A system for optically detecting at least one substance in a fluid, comprising:

at least one first agent, wherein:

the at least one first agent is reactive with the at least one substance, and

an optical response is produced during interaction of the at least one first agent and the at least one substance;

a chamber to contain the fluid, wherein:

the chamber is closed at one side by a window, and

the window is comprised of a material optically transparent to only a selected portion of a spectrum of incident wavelengths that includes a wavelength of the optical response, the selected portion including visible light wavelengths of only a single color spectrum;

first and second optical detectors, wherein the first and second optical detectors are arranged and adapted to each detect at least a portion of the optical response, the first optical detector vertically aligned with the window, and the second optical detector positioned differently than the first optical detector on one side of the window;

a first magnetic bead attached to the at least one first agent to form a particle, the particle stored in a dried assay prior to the fluid being provided to the chamber;

at least one magnetic source, wherein:

the at least one magnetic source is arranged to operate in a first mode and a second mode, the first mode for attraction of the first magnetic bead toward the window, and the second mode for repulsion of the first magnetic bead away from the window, and

only the at least one magnetic source allows the particle to be retained onto or close to a sensor surface extending between the window and the chamber; and

a processor circuit, wherein:

the processor circuit determines a quantity of the at least one substance in the fluid based on signals provided by the first and second optical detectors, and

the first and second optical detectors are each positioned to receive at least a portion of the optical response from the at least one first agent located upon or close to the window.

16. A method of optically detecting at least one substance in a fluid, comprising:

storing at least one particle comprising a magnetic bead and a first agent in a dried assay,

wherein the first agent is reactive with the at least one substance, and

wherein an optical response is produced during interaction of the first agent and the at least one substance;

providing a chamber with a window,

wherein the window is comprised of a material optically transparent to only a selected portion of a spectrum of incident wavelengths that includes a wavelength of the optical response and a wavelength of an optical excitation, the portion including visible light wavelengths of only a single color spectrum;

providing the at least one particle and the fluid in the chamber;

providing first and second optical detectors, wherein the first and second optical detectors are arranged and adapted to each detect at least a portion of the optical response, the first optical detector vertically aligned with the window, and the second optical detector positioned differently than the first optical detector on one side of the window;

providing at least one magnetic source having a first mode of operation and a second mode of operation, the first mode for attraction of the magnetic bead toward the window, and the second mode for repulsion of the magnetic bead away from the window;

magnetically moving the at least one particle onto or close to the window; and

magnetically retaining the at least one particle onto or close to the window while detecting at least a portion of the optical response from the at least one particle;

wherein only the at least one magnetic source allows the at least one particle to be retained onto or close to the window.