US 12,339,215 B2
Method for determining the viability of cells
Cedric Allier, Grenoble (FR); Lionel Herve, Grenoble (FR); Geoffrey Esteban, Teyran (FR); Martin Pisaneschi, Jacou (FR); and Melissa Hill, King of Prussia, PA (US)
Assigned to COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, Paris (FR); IPRASENSE SAS, Clapiers (FR); and GLAXOSMITHKLINE INTELLECTUAL PROPERTY DEVELOPMENT LIMITED, Stevenage (GB)
Appl. No. 17/995,775
Filed by COMMISSARIAT À L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, Paris (FR); IPRASENSE SAS, Clapiers (FR); and GLAXOSMITHKLINE INTELLECTUAL PROPERTY DEVELOPMENT LIMITED, Brentford (GB)
PCT Filed Apr. 8, 2020, PCT No. PCT/US2020/027263
§ 371(c)(1), (2) Date Oct. 7, 2022,
PCT Pub. No. WO2021/206707, PCT Pub. Date Oct. 14, 2021.
Prior Publication US 2023/0168180 A1, Jun. 1, 2023
Int. Cl. G01N 15/1433 (2024.01); C12M 1/34 (2006.01); G01N 15/14 (2006.01)
CPC G01N 15/1433 (2024.01) [C12M 41/46 (2013.01); G01N 2015/1488 (2013.01); G01N 2510/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for determining a state of a cell, the cell being placed in a sample in contact with a culture medium, the method comprising:
a) illuminating the sample with a light source and acquiring an image of the sample with an image sensor, the image sensor lying in a detection plane;
b) from the acquired image, locating a position of the cell in a plane parallel to the detection plane;
c) from the acquired image, estimating a refractive index of the cell or a relative refractive index of the cell, the relative refractive index corresponding to the refractive index of the cell relative to the refractive index of the culture medium;
d) from the estimation of the refractive index or of the relative refractive index, determining an index of interest of the cell; and
e) from the index of interest, classifying a state of the cell among predetermined states, the predetermined states comprising at least one apoptosis state and one living state,
wherein:
in c), the refractive index or the relative refractive index is a complex quantity;
in d), the index of interest is determined depending, on at least one of:
a real part of the refractive index or of the relative refractive index; and
an imaginary part of the refractive index or of the relative refractive index.