US 12,338,498 B2
High steviol glycoside-containing stevia plant and method for screening same
Tadayoshi Hirai, Kyoto (JP); Kazunari Iwaki, Kanagawa (JP); Kentaro Ochiai, Kyoto (JP); Saori Takeyama, Kanagawa (JP); and Katsuro Miyagawa, Kyoto (JP)
Assigned to SUNTORY HOLDINGS LIMITED, Osaka (JP)
Appl. No. 17/924,547
Filed by SUNTORY HOLDINGS LIMITED, Osaka (JP)
PCT Filed May 11, 2021, PCT No. PCT/JP2021/017956
§ 371(c)(1), (2) Date Nov. 10, 2022,
PCT Pub. No. WO2021/230259, PCT Pub. Date Nov. 18, 2021.
Claims priority of application No. 2020-084133 (JP), filed on May 12, 2020.
Prior Publication US 2023/0265530 A1, Aug. 24, 2023
Int. Cl. C12Q 1/6895 (2018.01); A01H 6/14 (2018.01); A23L 27/30 (2016.01)
CPC C12Q 1/6895 (2013.01) [A01H 6/1488 (2018.05); A23L 27/36 (2016.08); C12Q 2600/13 (2013.01)] 18 Claims
 
1. A method of screening for a high steviol glycoside-content stevia plant, comprising detecting from the genome of a test stevia plant the presence and/or the absence of the following genetic features (1), and the presence and/or the absence of the following genetic features (2):
(1) homozygous for the allele wherein the base at the position corresponding to position 290 of SEQ ID NO: 1 is T; and
(2) homozygous or heterozygous for the allele wherein the base at the position corresponding to position 40 of SEQ ID NO: 2 is A.