US 12,338,484 B2
FRET biosensor for detecting and reporting NAD+/NADH ratio changes
Grigory Filonov, Ann Arbor, MI (US); and Cicely Schramm, Ann Arbor, MI (US)
Assigned to Sartorius BioAnalytical Instruments, Inc., Bohemia, NY (US)
Filed by Sartorius BioAnalytical Instruments, Inc., Bohemia, NY (US)
Filed on Jul. 12, 2022, as Appl. No. 17/812,030.
Prior Publication US 2024/0026420 A1, Jan. 25, 2024
Int. Cl. C12Q 1/48 (2006.01); G01N 33/542 (2006.01)
CPC C12Q 1/485 (2013.01) [G01N 33/542 (2013.01)] 15 Claims
 
1. A polynucleotide encoding a fusion protein, wherein the fusion protein comprises the amino acid sequence selected from the group consisting of:
(a) residues 1-867 of SEQ ID NO: 13;
(b) residues 1-861 of SEQ ID NO: 14;
(c) residues 1-863 of SEQ ID NO: 15;
(d) residues 1-862 of SEQ ID NO:16;
(e) residues 1-865 of SEQ ID NO:20;
(f) residues 1-853 of SEQ ID NO:24;
(g) residues 1-868 of SEQ ID NO:191;
(h) residues 1-867 of SEQ ID NO:192; and
(i) residues 1-866 of SEQ ID NO:193.